GenScript - Vector - Based siRNA - Plasmidic Expression Vector

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GenScript Plasmidic siRNA Expression Vectors

These vectors are designed for mammalian transfection. They each carry a neomycin, hygromycin, or zeomycin resistance gene as a selectable marker, which can be used for establishing stable cell lines. The pRNAT vectors also carry a GFP marker (coral GFP or cGFP) under CMV promoter* control, which can be used to track transfection efficiency. They use regular or enhanced U6, or H1, or CMV promoters to drive siRNA expression.

Cat. No.	Vector	Promoter	Resistance	Marker	Map	Price
SD1201	pRNA-U6.1/Neo	U6	Neomycin	-	Map	$225
SD1202	pRNA-U6.1/Hygro	U6	Hygromycin	-	Map	$225
SD1207	pRNA-U6.1/Zeo	U6	Zeomycin	-	Map	$225
SD1203	pRNA-H1.1/Neo	H1	Neomycin	-	Map	$225
SD1204	pRNA-H1.1/Hygro	H1	Hygromycin	-	Map	$225
SD1208	pRNA-H1.1/Zeo	H1	Zeomycin	-	Map	$225
SD1231	pRNA-CMV3.1/Neo	CMV	Neomycin	-	Map	$225
SD1232	pRNA-CMV3.1/Hygro	CMV	Hygromycin	-	Map	$225
SD1233	pRNA-CMV3.1/Puro	CMV	Puromycin	-	Map	$225
SD1211	pRNAT-U6.1/Neo	U6	Neomycin	cGFP	Map	$350
SD1212	pRNAT-U6.1/Hygro	U6	Hygromycin	cGFP	Map	$350
SD1213	pRNAT-H1.1/Neo	H1	Neomycin	cGFP	Map	$350
SD1214	pRNAT-H1.1/Hygro	H1	Hygromycin	cGFP	Map	$350
SD1261	pRNAT-CMV3.1/Neo	CMV	Neomycin	cGFP	Map	$350
SD1262	pRNAT-CMV3.1/Hygro	CMV	Hygromycin	cGFP	Map	$350
SD1263	pRNAT-CMV3.1/Puro	CMV	Puromycin	cGFP	Map	$350
SD1264	pRNAT-CMV3.2/Neo	CMV	Neomycin	cGFP	Map	$350
SD1265	pRNAT-CMV3.2/Hygro	CMV	Hygromycin	cGFP	Map	$350
SD1266	pRNAT-CMV3.2/Puro	CMV	Puromycin	cGFP	Map	$350
SD1550	pRNA-U6.3/Neo	U6.3 (enhanced)	Neomycin	-	Map	$225
SD1552	pRNA-H1.3/Neo	H1.3 (enhanced)	Neomycin	-	Map	$225
SD1561	pRNAT-U6.3/Hygro	U6.3 (enhanced)	Hygromycin	cGFP	Map	$350
SD1563	pRNAT-H1.3/Hygro	H1.3 (enhanced)	Hygromycin	cGFP	Map	$350

To optimize the experimental parameters of DNA vector-based siRNA constructs, we have designed positive controls, which can knock down firefly luciferase and Renilla luciferase. These can be used for initial test experiments.

* Limited Use Label License: The use of CMV promoter is covered under U. S. Patents Nos. 5,168,062 and 5,385,839, owned and licensed by the University of Iowa Research Foundation and is sold for research use only. Commercial users must obtain a license to these patents directly from the University of Iowa Research Foundation (UIRF), 214 Technology Innovation Center, Iowa City, Iowa 52242. For further information, please contact the Associate Director of UIRF at 319-335-4546.