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siRNA Positive Controls

Firefly Luciferase siRNA Constructs: siFLuc

siFLuc inserts are siRNA constructs designed to knock down firefly luciferase. In GenScript's controlled experiments, this siRNA can knock down firefly luciferase activity by about 80%. The target sequence (CTTACGCTGAGTACTTCGA) was selected based on an article published in Nature Genetics (2002) 32:107. The target sequence matches the pGL3-control and is designed to silence firefly luciferase expressed by a co-transfected pGL3-control vector (Promega, Cat. #E1741). The alignment is as follows:

Target: 1 CTTACGCTGAGTACTTCGA 19 ||||||||||||||||||| pGL3-Control: 434 CTTACGCTGAGTACTTCGA 452


Firefly Luciferase siRNA Positive Controls

The siFLuc insert has been cloned into a pRNA series vector using BamH I and Hind III sites. It has been sequence-verified.

Cat. No.
Name
Description
Quantity
Price
SD1501
siRNA insert in pRNA-U6.1/Neo
10 µg
$225
SD1502
siRNA insert in pRNA-U6.1/Hygro
10 µg
$225
SD1503
siRNA insert in pRNA-H1.1/Neo
10 µg
$225
SD1504
siRNA insert in pRNA-H1.1/Hygro
10 µg
$225
SD1531
siRNA insert in pRNA-CMV3.1/Neo
10 µg
$225
SD1532
siRNA insert in pRNA-CMV3.1/Hygro
10 µg
$225
SD1512
siRNA insert in pRNAT-U6.1/Hygro (with cGFP)
10 µg
$225
SD1551
siRNA insert in pRNA-U6.3/Neo
10 µg
$225
SD1553
siRNA insert in pRNA-H1.3/Neo
10 µg
$225
SD1565
siRNA insert in pRNAT-U6.3/Hygro (with cGFP)
10 µg
$350
SD1566
siRNA insert in pRNAT-H1.3/Hygro (with cGFP)
10 µg
$350

siFLuc Positive Controls for GenScript Inducible siRNA Vectors:

Cat. No.
Name
Description
Quantity
Price
SD1523
siRNA insert in pRNATin-H1.2/Neo/siFLuc
10 µg
$225


Renilla Luciferase siRNA Constructs: siRLuc

Download: product information (PDF)

siRLuc are siRNA constructs designed to knock down Renill a luciferase activity. In our controlled experiments, this siRNA can knock down Renilla luciferase activity by about 80%. The target sequence (GTAGCGCGGTGTATTATAC) was selected based on an article published in Nature Biotechnology (2002), 20:497. The target sequence matches pRL-TK (Promega, Cat. #E2241) and is designed to silence Renilla luciferase expressed by a co-transfected pRL-TK vector. The alignment is as follows:

Target: 1 GTAGCGCGGTGTATTATAC 19 ||||||||||||||||||| pRL-TK: 1241 GTAGCGCGGTGTATTATAC 1259


Renilla Luciferase siRNA Positive Controls

The siRLuc insert has been cloned into a GenScript pRNA series vector using BamH I and Hind III sites. It has been sequence-verified.

Cat. No.
Name
Description
Quantity
Price
SD1601
siRNA insert in pRNA-U6.1/Neo
10 µg
$225
SD1602
siRNA insert in pRNA-U6.1/Hygro
10 µg
$225
SD1603
siRNA insert in pRNA-H1.1/Neo
10 µg
$225
SD1604
siRNA insert in pRNA-H1.1/Hygro
10 µg
$225



cGFP (Coral Green Fluorescence Protein) siRNA Constructs: si-cGFP

The si-cGFP controls are siRNA constructs designed to knock down cGFP (coral green fluorescent protein) expression. In our controlled experiments, this siRNA can knock down cGFP fluorescence by about 80%. The si-cGFP controls are easy to use and allow scientists to monitor the effects of siRNA by using fluorescence microscopes. The si-cGFP inserts have been cloned into pRNA-U6.1/Neo and pRNA-H1.1/Neo vectors. As such, they can be used to test the effects of both U6 and H1 promoters.

cGFP siRNA Positive Controls

Cat. No.
Name
Description
Quantity
Price
SD1701
si-cGFP insert in pRNA-U6.1/Neo
10 µg
$225
SD1702
si-cGFP insert in pRNA-H1.1/Neo
10 µg
$225

Please follow this link to order GenScript siRNA Negative controls.