siRNA Positive Controls
Firefly Luciferase siRNA
Constructs: siFLuc
siFLuc inserts are siRNA constructs designed to knock down
firefly luciferase. In GenScript's controlled experiments,
this siRNA can knock down firefly luciferase activity by about
80%. The target sequence (CTTACGCTGAGTACTTCGA) was selected
based on an article published in Nature Genetics (2002) 32:107. The target
sequence matches the pGL3-control and is designed to silence
firefly luciferase expressed by a co-transfected pGL3-control
vector (Promega, Cat. #E1741). The alignment is as follows:
| Target: 1 CTTACGCTGAGTACTTCGA 19
|||||||||||||||||||
pGL3-Control: 434 CTTACGCTGAGTACTTCGA 452
|
Firefly Luciferase siRNA Positive
Controls
|
The siFLuc insert has been cloned into a pRNA series
vector using BamH I and
Hind III sites. It has been
sequence-verified.
|
Cat. No.
|
Name
|
Description
|
Quantity
|
Price
|
|
SD1501
|
|
si-FLuc insert in pRNA-U6.1/Neo
|
10 µg
|
$225
|
|
SD1502
|
|
si-FLuc insert in pRNA-U6.1/Hygro
|
10 µg
|
$225
|
|
SD1503
|
|
si-FLuc insert in pRNA-H1.1/Neo
|
10 µg
|
$225
|
|
SD1504
|
|
si-FLuc insert in pRNA-H1.1/Hygro
|
10 µg
|
$225
|
|
SD1531
|
|
si-FLuc insert in pRNA-CMV3.1/Neo
|
10 µg
|
$225
|
|
SD1532
|
|
si-FLuc insert in pRNA-CMV3.1/Hygro
|
10 µg
|
$225
|
|
SD1512
|
|
si-FLuc insert in pRNAT-U6.1/Hygro (with cGFP)
|
10 µg
|
$225
|
|
SD1551
|
|
si-FLuc insert in pRNA-U6.3/Neo
|
10 µg
|
$225
|
|
SD1553
|
|
si-FLuc insert in pRNA-H1.3/Neo
|
10 µg
|
$225
|
|
SD1565
|
|
si-FLuc insert in pRNAT-U6.3/Hygro (with cGFP)
|
10 µg
|
$350
|
|
SD1566
|
|
si-FLuc insert in pRNAT-H1.3/Hygro (with cGFP)
|
10 µg
|
$350
|
siFLuc Positive Controls for
GenScript Inducible siRNA Vectors:
|
Cat. No.
|
Name
|
Description
|
Quantity
|
Price
|
|
SD1523
|
|
si-FLuc insert in pRNATin-H1.2/Neo
|
10 µg
|
$225
|
Renilla
Luciferase siRNA Constructs: siRLuc
Download: product information (PDF)
siRLuc are
siRNA constructs designed to knock down Renilla luciferase activity. In our controlled experiments, this siRNA can knock down Renilla luciferase
activity by about 80%. The target sequence
(GTAGCGCGGTGTATTATAC) was selected based on an article published in Nature Biotechnology (2002), 20:497. The
target sequence matches pRL-TK (Promega, Cat. #E2241) and is designed to silence Renilla
luciferase expressed by a co-transfected pRL-TK vector. The
alignment is as follows:
Target: 1 GTAGCGCGGTGTATTATAC 19
|||||||||||||||||||
pRL-TK: 1241 GTAGCGCGGTGTATTATAC 1259
|
Renilla Luciferase siRNA Positive
Controls
|
The siRLuc insert has been cloned into a GenScript pRNA
series vector using BamH I and
Hind III sites. It has been
sequence-verified.
|
Cat. No.
|
Name
|
Description
|
Quantity
|
Price
|
|
SD1601
|
|
si-RLuc insert in pRNA-U6.1/Neo
|
10 µg
|
$225
|
|
SD1602
|
|
si-RLuc insert in pRNA-U6.1/Hygro
|
10 µg
|
$225
|
|
SD1603
|
|
si-RLuc insert in pRNA-H1.1/Neo
|
10 µg
|
$225
|
|
SD1604
|
|
si-RLuc insert in pRNA-H1.1/Hygro
|
10 µg
|
$225
|
cGFP (Coral Green Fluorescence
Protein) siRNA Constructs: si-cGFP
The
si-cGFP controls are siRNA constructs designed to knock down
cGFP (coral green fluorescent protein) expression. In our
controlled experiments, this siRNA
can knock down cGFP fluorescence by about 80%. The si-cGFP
controls are easy to use and allow scientists to monitor the
effects of siRNA by using fluorescence microscopes. The
si-cGFP inserts have been cloned into pRNA-U6.1/Neo and
pRNA-H1.1/Neo vectors. As such, they can be used to test the
effects of both U6 and H1 promoters.
cGFP siRNA Positive Controls
|
Cat. No.
|
Name
|
Description
|
Quantity
|
Price
|
|
SD1701
|
|
si-cGFP insert in pRNA-U6.1/Neo
|
10 µg
|
$225
|
|
SD1702
|
|
si-cGFP insert in pRNA-H1.1/Neo
|
10 µg
|
$225
|
Please follow this link to order
GenScript siRNA
Negative controls.
|
