Adenoviral siRNA Vectors:

The following GenScript adenoviral vectors are compatible with the Stratagene AdEasy^TM Adenoviral Vector System. Each uses either an H1 or inducible H1 promoter to drive siRNA expression. The pRNAT vectors carry a GFP marker (coral GFP or cGFP) under CMV promoter* control, which can be used to track transfection efficiency.

Lentiviral siRNA Vectors:

GenScript Lentiviral siRNA expression vectors are compatible with the Invitrogen ViraPower^TM Lentiviral Expression System. They are designed for siRNA expression in both dividing and nondividing cells. Each vector contains a neomycin resistance gene for establishing stable cell lines and a coral GFP gene for tracking transfection efficiency, respectively driven by an SV40 promoter and a CMV promoter. The vector uses a Rous sarcoma virus (RSV) enhancer/promoter fused to HIV 5'LTR (PRSV/5'LTR) for viral transcription and HIV 3'LTR for packaging.

Retroviral siRNA Vectors:

GenScript Retroviral siRNA expression vectors are compatible with the Clontech Retro-X^TM Expression System. The siRNA insert is cloned between the Mli and Xho sites. An H1 promoter drives siRNA expression. The vector contains a hygromycin resistance gene under the control of a CMV promoter* for establishing stable cell lines. The vector uses MSV 3'LTR for viral transcription and a CMV enhancer/promoter joined to MSV 5'LTR (CMV/MSV 5'LTR) for packaging.