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All the activities are normalized by Firefly Luciferase actitivty. The activities come from:
  • pGL: HEK293 cells transfected with pGL3-control (0.16 ug) and pRL-TK (0.16 ug).
  • U6-siLuc: HEK293 cells transfected with pGL3-control (0.16 ug), pRL-TK (0.16 ug), and 1.6 ug of pRNA-U6.1/Neo/siRLuc.
  • H1-siLuc: HEK293 cells transfected with pGL3-control (0.16 ug), pRL-TK (0.16 ug), and 1.6 ug of pRNA-H1.1/Neo/siRLuc.
  • U6: HEK293 cells transfected with pGL3-control (0.16 ug), pRL-TK (0.16 ug), and 1.6 ug of pRNA-U6.1/Neo empty vector.




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