One-Step WesternTM Basic Kits
One-Step WesternTM Basic Kits are the simplified version of One-Step WesternTM Complete Kits. The WestClearTM
Nitrocellulose Membrane and LumiSensorTM Chemiluminescent HRP Substrate Kit and X-Ray film are not included in the package.
It is economical to choose these kits if you have surplus accessories in hand. Associated with GenScript's
accessories, One-Step WesternTM Basic Kits can perform as excellent as One-Step WesternTM Complete Kits.
To obtain best results and reduce undesirable variations such as high background, we strongly recommend that the following reagents be used with One-Step WesternTM Basic Kit:
- GenScript WestClearTM Nitrocellulose Membrane (L00224A60, L00224A225).
- GenScript LumiSensorTM Chemiluminescent HRP Substrate Kit (L00221V60).
- GenScript ChromoSensorTM One Solution TMB Substrate (L00222V60).
Key Features:
- Easy to perform: Fewer steps mean fewer chances for human error.
- High sensitivity: The kit's sensitivity is comparable to or even better better than that of the classical 4.5-hour procedure, depending on the quality and amount of antibodies used.
- Reproducible results: The kit produces highly reproducible results.
- Far less optimization than the classical three-step method.
- Secondary antibody is included.
Applications:
- Protein/antigen detection
- Evaluation of protein expression
- Antibody/antigen titration
- Analysis and comparison of primary antibody activity levels
Technical Manual:
Download PDF file TM0352
Product List:
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