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One-Step WesternTM Complete Kit
| Developed using our proprietary One-Step WesternTM technology, One-step WesternTM
Complete Kit reduces the total analysis time for a Western blot or Dot
blot to just one hour. The kit contains all the necessary reagents,
buffers, nitrocellulose membrane and HRP substrate for performing
western blot, and compatible with both chemiluminescent substrates and
chromogenic substrates, such as DAB and TMB. |
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Key Features:
- Easy to perform: Fewer steps mean fewer chances for human error.
- High sensitivity: The
sensitivity of the kit is comparable to or better than that of the
classical 4.5-hour procedure, depending on the quality and amount of
antibodies used.
- Reproducible results: The kit produces highly reproducible results.
- Needs far less optimization than the classical three-step method.
- No additional secondary antibody is needed.
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Applications:One-Step WesternTM Kit cuts hours off the following applications. One-Step WesternTM Complete Kit provides everything for such operation:
- Protein/antigen detection
- Evaluation of protein expression
- Antibody/antigen titration
- Analysis and comparison of primary antibody activity levels
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Example: |
1. Western blot detection of housekeeping protein α-tubulin.
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Figure 2. Western blot detection of α-Tubulin using the One-Step WesternTM
Kit (Cat. No. L00204C) and α-tubulin polyclonal antibody (Abcam,
ab4074). 10 μg, 5.0 μg, 2.5 μg, 1.25 μg, 0.62 μg, 0.31 μg, 0.16 μg, and
0.08 μg of Hela cell lysates (BD Biosciences, 611449) were loaded into
Lane 1, Lane 2, Lane 3, Lane 4, Lane 5, Lane 6, Lane 7, and Lane 8,
respectively. The blot was developed with the LumiSensorTM Chemiluminescent HRP Substrate included in the kit. |
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