Stable cell lines are used in a variety of research applications like recombinant protein and antibody production, assay development, gene editing, and functional studies etc. At GenScript, we offer comprehensive stable cell line development capabilities that either begins with DNA provided by our clients or that are powered by our fast, high efficiency gene synthesis service that seamlessly integrates all steps of stable cell line generation, delivering high quality stable clones of your choice. Boost your productivity and improve your results by leveraging GenScript’s top quality stable cell line services.
We offer GLP compliant services in both these categories
Flow chart of our Stable Cell Line Development Process
High quality cell lines start with high quality DNA
Our customers have the option of providing their own DNA for stable cell line generation purposes, however, GenScript recommends gene synthesis to ensure that we use high quality, codon optimized gene for the best expression levels.
To create stable cell lines, DNA carrying the gene of interest has to be introduced into the cell. GenScript allows you to choose either plasmid-based transfection or lentivirus infection to deliver the DNA into the cells.
Transient expression evaluation
To ensure that your protein of interest is expressed in the cells, we first characterize your protein expression by ELISA or Western Blot 48 hours after the transfection.
Stable cell line development
Transfected cells are grown in the appropriate selective media to generate stable pools. We can also subject the stable pools to additional Methotrexate (MTX) amplification and screening, in order to increase expression levels. The productivity and quality of the pools is evaluated up to 10 passages for the top 10 clones.
Top 3 clones from each pool are picked and evaluated further, based on their growth rates.
Stability testing is a critical component of characterizing bioproduction cell lines. Stable cell lines in continuous culture suffer from genetic instability as the number of passages increases. Stability test will help you ensure the quality of your production cells over the intended passages.
Final deliverables, QC data and full report
We provide the sequence and construct report (in case of synthesized gene), top 3 stable clones and a comprehensive stable cell line generation report
For any other inquiries, please contact us using the details listed below. Our customer service representatives are available 24 hours a day, Monday through Friday to assist you.