Stable Cell Line Development Services
GenScript's stable cell line service covers all your needs in the aspects of protein/antibody production, and assay development. Not only have we developed cell lines stable at expression level, but also established stable cell lines ready for assay development. Furthermore, GenScript's CellPowerTM platform guarantees over-expressing of gene of interest in any cell hosts. Outline of Stable Cell Line Services
- Research Cell Line
- Production Cell Line
- Guaranteed Cell Line
- Cell Line for Assays
Stable Research Cell Line (SC1260) | Services | Description | Deliverables | Timeline | | 1. Gene synthesis & Subcloning (Optional) | | - Sequence report
- Construct(s) report
| 4 weeks | | 2.Transient expression evaluation | - Transient expression analysis
- Detect the expression level by ELISA/WB/Flow cytometry/RT-PCR and etc
| | 1–2 weeks | | 3.Cell line development using mammalian cells | - Stable transfection and screening
- 10 passages evaluation for top 10 clones (Optional)
- Deliver 3 vials each of top 3 clones
| - Top 3 stable clones
- Report
- QC data
| 9–11 weeks | | Stable Production Cell Line (SC1503) | Services | Description | Deliverables | Timeline | | 1. Gene synthesis & Subcloning (Optional) | | - Sequence report
- Construct(s) report
| 4 weeks | 2.Transient expression
evaluation | - Transient expression analysis
- Detect the expression level by ELISA,WB and etc
| | 2–3 weeks | | 3.Stable cell line development using mammalian cells | - Stable transfection and screening
- MTX amplification and screening
- 10 passages evaluation for top 10 clones
- Deliver 3 vials each of top 3 clones
| - Top 3 stable clones
- Report
- QC data
| 21–26 weeks | Stable Industrial Cell Line (SC1557) | Services | Description | Deliverables | Timeline | | 1. Gene synthesis & Subcloning (Optional) | | - Sequence report
- Construct(s) report
| 4 weeks | 2.Transient expression
evaluation | - Transient expression analysis
- Detect the expression level by ELISA,WB and etc
| | 2–3 weeks | | 3.Stable cell line development using mammalian cells | - Stable transfection and screening
- MTX amplification and screening
- 10 passages evaluation for top 10 clones
- Deliver 3 vials each of top 5 clones
| - Top 3 stable clones
- Report
- QC data
| 21–26 weeks | | 4.Upstream process development | - Medium selection
- Physical compatibility experiment
- Feed supplementation test
| | 8-10 weeks |
Case Study  | | Fig. MTX amplification for CHO cells expressing a Fc fusion protein. The expression level of the Fc fusion protein increases more than 40-fold after 3 rounds of genomic amplification using MTX. | |
Service package(SC1394A & SC1394B)
| Service Steps | Service Features |
Additional Validation Services |
Subcloning of gene of interest |
Most affordable price
- $2490 for stable pool
- $4990 for single cell-derived clones
|
Membrane receptors: FACS, ELISA & Western Blot | Plasmid preparation |
GPCRs: Ca2+, cAMP, IP-1, radiometric binding assays |
Lentivirus packaging and production | Ion Channels: electrophysiology,
Transporters: radiometric assay | Transduction & selection |
Fast turnaround
- 8-10 weeks for stable pool
- 12-14 weeks for single cell-derived clones
| Proteases, kinases, and other enzyme:
substrate conversion assay | Q-PCR validated single(poly)-clone | Nuclear receptors: reporter gene assays | Deliverables: 2 vials of cells,
3X106 cells each vial | Cytokines: proliferation or other relevant assays | GenScript's promises: Delivery or no fee Mycoplasma-free cells guaranteed Any genes and any host cell lines customized with your own project 100% success of developing over 200 stable cell lines in the past 4 years Guaranteed expression of your genes validated by specific screening methods designated for your proteins |
Service Package(SC1394):
GenScript utilizes 2-round selection strategy for GPCR and ion channel stable cell line generation upon different applications. To reduce the cost and timeline, GenScript chooses HTS-compatible fluorescence- or luminescence-based assays to screen stable clones in the 1st round selection. In 2nd round selection, radioisotope- and electrophysiology-based assays are available upon your applications. For nuclear receptors, transporters, and other targets which have no ready HTS-compatible assays, GenScript offers mRNA or protein level assays as alternative solutions.
In addition, GenScript can develop a new assay upon your specifications. Please feel free to contact us for technique support.
Target Class | Type | 1st Round Selection | 2nd Round Selection | | GPCRs | Gq-coupled | Calcium assay | Radioligand binding assay | | Gi/Gs-coupled | Calcium assay (Gqi5/Gα15), cAMP assay, or luciferase reporter gene assay | | Ion Channels | Calcium channels | Calcium assay | Radioligand binding assay or manual patch clamp assay | | Potassium channels | Thallium flux assay or membrane potential assay | | Sodium channels | Membrane potential assay | | Chloride channels | YFP reporter gene assay | Nuclear Receptors, Transporters, etc. | | Upon client's specifications | Upon client's specifications |
Note:
CHO-K1, CHO-K1/Gα15, CHO-K1/Gqi5, HEK293, 293T, 293EC18, 293EC18/CRE-Luc, 1321N1, RH7777, and U-2OS are available as starting cell lines. For special requirements, please feel free to contact us.
For case studies and quotation, please refer to following website: Stable Cell Line for Assay Development
| Quotations and Ordering:  To request a quotation, please download and complete the Quotation Form, and send it back by email or fax.
- For our protein production mammalian stable cell line development service, please download
 . - For our guaranteed stable cell line development service, please give us a
Or refer to Guaranteed Stable Cell Line Service To order, please send the completed Quotation Form to us by email or fax with a formal PO (Purchase Order) or credit card information. You can also submit PO/credit card information by phone or via our secure online messaging system. If you are also submitting samples, please mail them together with a hard copy of the completed Quotation Form to the following address: BioProcess Services, GenScript, 860 Centennial Avenue., Piscataway, NJ 08854, US. Our customer service representatives are available 24 hours a day, Monday through Friday to assist you.
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