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Stable cell line generation

Stable Cell Line Development Services


GenScript's stable cell line service covers all your needs in the aspects of protein/antibody production, and assay development. Not only have we developed cell lines stable at expression level, but also established stable cell lines ready for assay development. Furthermore, GenScript's CellPowerTM platform guarantees over-expressing of gene of interest in any cell hosts.

Outline of Stable Cell Line Services

  • Research Cell Line
  • Production Cell Line
  • Guaranteed Cell Line
  • Cell Line for Assays

Stable Research Cell Line (SC1260)

Services Description Deliverables Timeline
1. Gene synthesis & Subcloning (Optional)
  • Sequence report
  • Construct(s) report
4 weeks
2.Transient expression evaluation
  • Transient expression analysis
  • Detect the expression level by ELISA/WB/Flow cytometry/RT-PCR and etc
  • Report
1–2 weeks
3.Cell line development using mammalian cells
  • Stable transfection and screening
  • 10 passages evaluation for top 10 clones (Optional)
  • Deliver 3 vials each of top 3 clones
  • Top 3 stable clones
  • Report
  • QC data
9–11 weeks

Stable Production Cell Line (SC1503)

Services Description Deliverables Timeline
1. Gene synthesis & Subcloning (Optional)
  • Sequence report
  • Construct(s) report
4 weeks
2.Transient expression
evaluation
  • Transient expression analysis
  • Detect the expression level by ELISA,WB and etc
  • Report
2–3 weeks
3.Stable cell line development using mammalian cells
  • Stable transfection and screening
  • MTX amplification and screening
  • 10 passages evaluation for top 10 clones
  • Deliver 3 vials each of top 3 clones
  • Top 3 stable clones
  • Report
  • QC data
21–26 weeks

Stable Industrial Cell Line (SC1557)

Services Description Deliverables Timeline
1. Gene synthesis & Subcloning (Optional)
  • Sequence report
  • Construct(s) report
4 weeks
2.Transient expression
evaluation
  • Transient expression analysis
  • Detect the expression level by ELISA,WB and etc
  • Report
2–3 weeks
3.Stable cell line development using mammalian cells
  • Stable transfection and screening
  • MTX amplification and screening
  • 10 passages evaluation for top 10 clones
  • Deliver 3 vials each of top 5 clones
  • Top 3 stable clones
  • Report
  • QC data
21–26 weeks
4.Upstream process development
  • Medium selection
  • Physical compatibility experiment
  • Feed supplementation test
  • Report
8-10 weeks

Case Study

MTX amplification and screening  
Fig. MTX amplification for CHO cells expressing a Fc fusion protein. The expression level of the Fc fusion protein increases more than 40-fold after 3 rounds of genomic amplification using MTX.

Service package(SC1394A & SC1394B)

Service Steps Service Features Additional Validation Services

Subcloning of gene of interest

    Most affordable price
  • $2490 for stable pool
  • $4990 for single cell-derived clones

Membrane receptors: FACS, ELISA & Western Blot

Plasmid preparation

GPCRs: Ca2+, cAMP, IP-1, radiometric binding assays

Lentivirus packaging and production

Ion Channels: electrophysiology,
Transporters: radiometric assay

Transduction & selection

    Fast turnaround
  • 8-10 weeks for stable pool
  • 12-14 weeks for single cell-derived clones

Proteases, kinases, and other enzyme:
substrate conversion assay

Q-PCR validated single(poly)-clone

Nuclear receptors: reporter gene assays

Deliverables: 2 vials of cells,
3X106 cells each vial

Cytokines: proliferation or other relevant assays

GenScript's promises:

  • Delivery or no fee
  • Mycoplasma-free cells guaranteed
  • Any genes and any host cell lines customized with your own project
  • 100% success of developing over 200 stable cell lines in the past 4 years
  • Guaranteed expression of your genes validated by specific screening methods designated for your proteins
  • Refer to Guaranteed Stable Cell Line Service for more details

    Service Package(SC1394):

    GenScript utilizes 2-round selection strategy for GPCR and ion channel stable cell line generation upon different applications. To reduce the cost and timeline, GenScript chooses HTS-compatible fluorescence- or luminescence-based assays to screen stable clones in the 1st round selection. In 2nd round selection, radioisotope- and electrophysiology-based assays are available upon your applications. For nuclear receptors, transporters, and other targets which have no ready HTS-compatible assays, GenScript offers mRNA or protein level assays as alternative solutions.

    In addition, GenScript can develop a new assay upon your specifications. Please feel free to contact us for technique support.

    Target Class
    Type
    1st Round Selection
    2nd Round Selection
    GPCRs Gq-coupled Calcium assay Radioligand binding assay
    Gi/Gs-coupled Calcium assay (Gqi5/Gα15), cAMP assay, or luciferase reporter gene assay
    Ion Channels Calcium channels Calcium assay Radioligand binding assay or manual patch clamp assay
    Potassium channels Thallium flux assay or membrane potential assay
    Sodium channels Membrane potential assay
    Chloride channels YFP reporter gene assay
    Nuclear Receptors, Transporters, etc.
      Upon client's specifications Upon client's specifications
    Note:

    CHO-K1, CHO-K1/Gα15, CHO-K1/Gqi5, HEK293, 293T, 293EC18, 293EC18/CRE-Luc, 1321N1, RH7777, and U-2OS are available as starting cell lines. For special requirements, please feel free to contact us.
    For case studies and quotation, please refer to following website: Stable Cell Line for Assay Development


    Quotations and Ordering:

    To request a quotation, please download and complete the Quotation Form, and send it back by email or fax.

    • For our protein production mammalian stable cell line development service, please download .
    • For our guaranteed stable cell line development service, please give us a CellPower Service Quotation
      Or refer to Guaranteed Stable Cell Line Service

    To order, please send the completed Quotation Form to us by email or fax with a formal PO (Purchase Order) or credit card information. You can also submit PO/credit card information by phone or via our secure online messaging system.

    If you are also submitting samples, please mail them together with a hard copy of the completed Quotation Form to the following address: BioProcess Services, GenScript, 860 Centennial Avenue., Piscataway, NJ 08854, US.

    Our customer service representatives are available 24 hours a day, Monday through Friday to assist you.