Knock-In and Knock-Out Vector Construction Service

The construction of reliable targeting vectors is a prerequisite for the generation of genetically engineered animals. GenScript's advanced molecular biology platform has provided more than 160 targeting vectors to customers worldwide. These targeting vectors have been successfully used in traditional and conditional knock-out, knock-in, and many other genetic modification projects.

Key Features:

• PCR amplification up to 20 kb
• De novo gene synthesis-based quick cloning techniques
• Efficient target vector construction
• Time- and cost- effective

Service Specifications:

1. Vector construction for traditional knock-out (Conventional knock-out)

   Traditional knock-out involves deleting or disabling both copies of a specific gene. This can be highly advantageous for both biomedical research and drug development. GenScript has developed several powerful technology platforms for efficient and precise generation of most standard and conditional knock-out targeting vectors, by combining de novo gene synthesis and quick cloning techniques.

   Special promotion: Our standard service starting at $2,800, if sufficient BAC DNA is provided containing 3' and 5' homolog arms (≤ 4 kb) and free of complex sequences (repeats, high or low GC content), and destination vector supplied is commercially available.

2. Vector construction for conditional knock-out (Tissue-specific or developmental gene targeting)
   

   Conditional knock-out involves deleting or disabling a gene in only a particular organ, tissue, or cell type or only during a certain development stage. GenScript provides targeting vectors that can be tailored for inactivation of specific genes in specific tissues in a temporally specific manner. Our scientists have years of experience in inserting loxP and FRT sequences and modified DNA sequences into customized targeting vectors.

3. Vector construction for knock-in

   Reporter gene knock-in provides a more predictable expression pattern than random integration (transgenic methods). Similar to its conventional knock-out service, GenScript offers targeting vectors for efficient and precise genetic engineering with a variety of useful traits, such as site-specific modifications in specific genes and reporter gene knock-in.

4. Modification of targeting vector or BAC construction

   GenScript has established several simple and effective methods for the modification of plasmids and BACs, allowing precise engineering, unlimited cloning and subcloning, and mutagenesis of DNA molecules of any size.

Delivery Specifications:

The turnaround time for targeting vector projects is usually less than four weeks. Our typical delivery includes the following:

• 4 µg targeting vectors
• Restriction enzyme digestion map of the vectors
• Sequencing data covering the junction regions
• Sequencing data covering the arms

• 100 µg targeting vectors
• Linear targeting vectors
• Targeting vectors with ultra-low endotoxin

Quotations and Ordering:

You may contact us for quotation requests by phone or fax listed below. However, we recommend that you send your quotation requests via our secure online messaging system or by email at gene@genscript.com. Our customer service representatives are available 24 hours, Monday through Friday to assist you.

Please submit your plasmid DNA or stab cultures of the respective clones with detailed project requirements to Knock-In and Knock-Out Vector Construction, GenScript Corp., 120 Centennial Ave., Suite 105, Piscataway, NJ08854, US.

	Order by email: gene@genscript.com
	Order by phone: 1-877-436-7274 (Toll-Free) 1-732-885-9188
	Order by fax: 1-732-210-0262 1-732-885-5878