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Mammalian Expression System

Transient mammalian protein expression services

Recombinant protein production in eukaryotic cells has been essential in advancing our understanding of the functional significance of genes. Transient expression in mammalian cells is especially crucial when post-translational modifications and appropriate protein folding is desired for downstream applications. GenScript's transient mammalian protein expression service offers fast production and delivery of high quality recombinant proteins and monoclonal antibodies at very flexible scale, from milligram to gram quantities.

Flow chart of our Transient Expression services

#required for the guaranteed packages but optional for Custom services

We use Chinese Hamster Ovary (CHO) and Human Embryonic Kidney 293 (HEK) as our primary cell lines for transient expression owing to the ease of handling, robust growth rates, excellent transfectability and high yield recombinant protein-generation capacity.  If you are not sure if transient expression is right for you or if you are exploring a lower-cost mammalian protein expression evaluation package, we would recommend our PROTential™ Standard service so you can identify the best transient expression system for your target protein and identify the construct and conditions that give you the most robust, soluble transient expression of your target protein.  Alternately, if you are interested in generating a Stable cell line for your recombinant antibody or protein production, please visit our Stable cell line development page.

Package details of our Transient Expression services

Expression Package
Features
Timeline
Price
When to use this service
Few downstream Applications of protein
  • Starts with codon optimization & gene synthesis
  • Guaranteed service
  • Deliverables include synthetic gene, 3mg protein at 85% purity & QC data*
8-12 weeks
$8900
When you need hassle-free, high quality, synthetic gene and guaranteed transient mammalian protein expression
more details

(using downloadable inquiry form)
  • Starts with codon optimization & gene synthesis
  • Guaranteed service
  • Deliverables include synthetic gene, 50mg antibody & QC data*
8-12 weeks
$8499
When you need hassle-free, high quality, synthetic gene and guaranteed antibody transient expression
more details

(using downloadable inquiry form)
  • Starts with either customer-supplied construct or codon optimization & gene synthesis
  • Fully customizable service
  • Deliverables include protein as requested & QC data*
Starts at $2000
Flexible package when you need custom-scale services. Ideal if you have specific instructions or special preferences [e.g., cell line, protein purity levels, tags etc.]
more details
*QC data: SDS-PAGE will be performed to determine protein purity, A280 to measure protein concentration and MALDI-TOF peptide mapping to determine protein identity (if necessary)

Quotations and Ordering

For any other inquiries, plesae contact us using the details listed below. Our customer service repsentatives are available 24 hours a day, Monday through Friday, to assist you.