Recombinant protein production in eukaryotic cells has been essential in advancing our understanding of the functional significance of genes. Transient expression in mammalian cells is especially crucial when post-translational modifications and appropriate protein folding is desired for downstream applications. GenScript transient expression services offer rapid production and delivery of high quality proteins and recombinant antibodies (rAbs) from microgram to gram quantities.
Transient Expression Services Workflow
We use Chinese Hamster Ovary (CHO) and Human Embryonic Kidney 293 (HEK) as our primary cell lines for transient expression owing to the ease of handling, robust growth rates, excellent transfectability and high yield recombinant protein-generation capacity.
Transient Expression Service Selection Guide
For all other protein and rAb target types we offer fully customizable transient expression services in consultation with clients.
*QC data: SDS-PAGE and/or HPLC will be performed to determine protein and rAb purity. BCA or Bradford assay will be used to measure protein concentration and A280 will be used to measure rAb concentration. LC-MS/MS peptide mapping will be used to determine protein identity if necessary. GenScript uses The Endosafe®-PTS™ endotoxin testing system from Charles River Laboratories, for rapid endotoxin detection.
“Guarantee” indicates that deliverables will meet or exceed requirements listed for the respective service. If deliverables do not meet guarantee, there is no charge to customer.
If you are not sure if transient expression is right for you or if you are exploring a lower-cost mammalian protein expression evaluation package, we would recommend our PROTential™ Standard service so you can identify the best transient expression system for your target protein and identify the construct and conditions that give you the most robust, soluble transient expression of your target protein. Alternately, if you are interested in generating a Stable cell line for your rAb or protein production, please visit our Stable cell line development page.
Our customer service repsentatives are available 24 hours a day, Monday through Friday, to assist you.