Home » Recombinant Protein & rAb Services » Transient Expression
Email to GenScript

Mammalian Transient Expression System
Transient Expression Service Quotation

Mammalian Transient Expression Services

Recombinant protein production in eukaryotic cells has been essential in advancing our understanding of the functional significance of genes. Transient expression in mammalian cells is especially crucial when post-translational modifications and appropriate protein folding is desired for downstream applications. GenScript's transient mammalian protein expression service offers fast production and delivery of high quality recombinant proteins and monoclonal antibodies at very flexible scale, from microgram to gram quantities.

Flow chart of our Transient Expression services

Transient Expression services flowchart
# Required for the guaranteed packages but optional for Custom services

We use Chinese Hamster Ovary (CHO) and Human Embryonic Kidney 293 (HEK) as our primary cell lines for transient expression owing to the ease of handling, robust growth rates, excellent transfectability and high yield recombinant protein-generation capacity.

Package details of our Transient Expression services

Expression Package
Features
Timeline
Price
When to use this service
Few downstream Applications of protein
  • Starts with codon optimization & gene synthesis
  • Guaranteed service
  • Deliverables include synthetic genes, optimized gene sequence report, antibody at listed amount and purity, QC data
As soon as 5 weeks
Transient Expression Service Quotation
When you need hassle-free, high quality, synthetic gene and guaranteed antibody transient expression
more details
  • Starts with either codon optimization and gene synthesis or using customer-supplied DNA
  • Deliverables include affinity purified Ab, report & QC data
As soon as 3 weeks
High Throughput Antibody Purification Services Quotation
For transient recombinant antibody production of samples 24 and up. Get up to 1mg of recombinant antibody in just 3 weeks to accelerate your antibody development project
more details
  • Starts with codon optimization & gene synthesis
  • Guaranteed service
  • Deliverables include synthetic gene, target protein at listed amount & purity, QC data and final report
9-11 weeks
Transient Expression Service Quotation
When you need hassle-free, high quality, synthetic gene and guaranteed transient mammalian protein expression
more details
  • Starts with codon optimization and gene synthesis or using customer's expression construct
  • Flexible scale customizable service
  • Deliverables include synthetic gene (optional), expression report and purified protein
As soon as 5 weeks
Transient Expression Service Quotation
Ideal when you need to build your own, flexible scale mammalian expression projects
more details

*QC data: SDS-PAGE will be performed to determine protein purity. BCA or Bradford assay will be used to measure protein concentration and A280 will be used to measure antibody concentration. MALDI-TOF peptide mapping will be used to determine protein identity (if necessary)

If you are not sure if transient expression is right for you or if you are exploring a lower-cost mammalian protein expression evaluation package, we would recommend our PROTential™ Standard service so you can identify the best transient expression system for your target protein and identify the construct and conditions that give you the most robust, soluble transient expression of your target protein.  Alternately, if you are interested in generating a Stable cell line for your recombinant antibody or protein production, please visit our Stable cell line development page.

Transient Expression Service Quotation

Quotations and Ordering


Transient Expression services Quotations & Ordering

Our customer service repsentatives are available 24 hours a day, Monday through Friday, to assist you.