Recombinant protein production in eukaryotic cells has been essential in advancing our understanding of the functional significance of genes. Transient expression in mammalian cells is especially crucial when post-translational modifications and appropriate protein folding is desired for downstream applications. GenScript's transient mammalian protein expression service offers fast production and delivery of high quality recombinant proteins and monoclonal antibodies at very flexible scale, from microgram to gram quantities.
Flow chart of our Transient Expression services
We use Chinese Hamster Ovary (CHO) and Human Embryonic Kidney 293 (HEK) as our primary cell lines for transient expression owing to the ease of handling, robust growth rates, excellent transfectability and high yield recombinant protein-generation capacity. If you are not sure if transient expression is right for you or if you are exploring a lower-cost mammalian protein expression evaluation package, we would recommend our PROTential™ Standard service so you can identify the best transient expression system for your target protein and identify the construct and conditions that give you the most robust, soluble transient expression of your target protein. Alternately, if you are interested in generating a Stable cell line for your recombinant antibody or protein production, please visit our Stable cell line development page.
Package details of our Transient Expression services
*QC data: SDS-PAGE will be performed to determine protein purity. BCA or Bradford assay will be used to measure protein concentration and A280 will be used to measure antibody concentration. MALDI-TOF peptide mapping will be used to determine protein identity (if necessary)
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