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Mammalian Transient Expression System
Transient Expression Service Quotation

Transient Expression Services

Recombinant protein production in eukaryotic cells has been essential in advancing our understanding of the functional significance of genes. Transient expression in mammalian cells is especially crucial when post-translational modifications and appropriate protein folding is desired for downstream applications. GenScript transient expression services offer rapid production and delivery of high quality proteins and recombinant antibodies (rAbs) from microgram to gram quantities.

Transient Expression Services Workflow

Transient Expression services flowchart
# Required for the guaranteed packages but optional for Custom services

We use Chinese Hamster Ovary (CHO) and Human Embryonic Kidney 293 (HEK) as our primary cell lines for transient expression owing to the ease of handling, robust growth rates, excellent transfectability and high yield recombinant protein-generation capacity.

Transient Expression Service Selection Guide

Target Standardized Expression Packages When to use this service Features* Timeline Price
rAb
  • For rAb production of samples 24 and up, up to mg.
  • Starting under $400/rAb.
    more details
  • Starts with either codon optimization and gene synthesis or using customer-supplied DNA
  • Deliverables include affinity purified rAb, report & QC data
As soon as
3 weeks
High Throughput Antibody Purification Services Quotation
  • For rAb production up to 500mg at ≥95% purity.
  • Starting under $3000.
    more details
  • Starts with codon optimization & gene synthesis
  • Guaranteed service
  • Deliverables include synthetic genes, optimized gene sequence report, rAb, QC data
As soon as
5 weeks
Transient Expression Service Quotation
  • For large scale rAb production up to 5g at ≥99% purity.
    more details
  • Starts with codon optimization & gene synthesis
  • Guaranteed service
  • Deliverables include purified rAb, report, QC data
9-12 weeks
Transient Expression Service Quotation
Protein
  • For transient expression of secreted proteins and extracellular domains of membrane proteins.
  • Starting under $5000. more details
  • Starts with codon optimization & gene synthesis
  • Guaranteed service
  • Deliverables include synthetic gene, target protein, QC data and final report
9-11 weeks
Transient Expression Service Quotation
  • For transient expression of secreted proteins.
  • Starting under $2000.
    more details
  • Starts with codon optimization and gene synthesis or using customer's expression construct
  • Flexible scale customizable service
  • Deliverables include synthetic gene (optional), expression report and purified protein
As soon as
5 weeks
Transient Expression Service Quotation

For all other protein and rAb target types we offer fully customizable transient expression services in consultation with clients.
Write to us to discuss project details.

*QC data: SDS-PAGE will be performed to determine protein purity. BCA or Bradford assay will be used to measure protein concentration and A280 will be used to measure rAb concentration. MALDI-TOF peptide mapping will be used to determine protein identity (if necessary).

“Guarantee” indicates that deliverables will meet or exceed requirements listed for the respective service. If deliverables do not meet guarantee, there is no charge to customer.

If you are not sure if transient expression is right for you or if you are exploring a lower-cost mammalian protein expression evaluation package, we would recommend our PROTential™ Standard service so you can identify the best transient expression system for your target protein and identify the construct and conditions that give you the most robust, soluble transient expression of your target protein.  Alternately, if you are interested in generating a Stable cell line for your rAb or protein production, please visit our Stable cell line development page.

Transient Expression Service Quotation

Quotations and Ordering


Transient Expression services Quotations & Ordering

Our customer service repsentatives are available 24 hours a day, Monday through Friday, to assist you.