Mammalian Cell Expression System (SC1259)
Mammalian cells have the ability to perform most comprehensive post-translational modifications and to secrete glycoproteins that are correctly folded and contain complex antennary oligosaccharides with terminal sialic acid. Mammalian cells are widely used in the production of recombinant proteins, antibodies, virus, viral-subunit proteins, and gene-therapy vectors.
GenScript recommends its mammalian expression services for cases in which the folding of the proteins is crucial to the customer's intended application and cases in which the internal machinery of E. coli is unable to express the protein with desired conformation and modification. Our proprietary 293 and CHO suspension system platform promotes fast production and delivery of recombinant proteins and monoclonal antibodies up to grams level.
- Service Specifications
- Competitive Advantages
- Case Study
- Related Services
| Services |
Description |
Deliverables |
Timeline |
| 1. Subcloning (Optional) |
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2 weeks (Time for gene synthesis is not included) |
| 2. Small-scale protein expression |
- Pilot expression analysis and optimization
- Small-scale expression from one to five liters
- If the frozen cell line is supplied by the customer, we will revive the cells and raise them in an enlarged cell culture. (Need extra 1–2 weeks)
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2–3 weeks |
| 3. Protein purification and characterization |
- Protein purification by affinity column, ion exchange, gel filtration, or hydrophobic column
- Characterization by Western blot, SDS-PAGE, or sequencing
- Removal of tags and multi-step purification (Optional, additional charge and time can apply)
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- Purified proteins
- QC data
- Documentation of procedures
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2 weeks |
| 4. Large-scale protein production (Optional) |
- Harvest
- Testing of expression by Western blot
- Large-scale production
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Quote |
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1. 293TGETM Transient Expression Technology: HEK293TGE cell can be quickly adapted in large-scale suspension culture and protein of interests can be expressed/secreted at high level.
We also provide 15N- or 13C-labeled protein expression.
2. Large-scale Production and Purification: Recombinant protein and monoclonal antibody production capacity ranging from milligrams to gram level.
3. OptimumGeneTM Codon Optimization Technology: Our proprietary OptimumGeneTM codon optimization technology comprehensively optimizes factors critical to transcription, translation, and co-translational protein folding to deliver the highest possible levels of expression in any system.
4. One-stop Services: GenScript can take your project directly from
gene synthesis, to protein expression, stable cell line generation, antibody production and assay development.
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1. Monoclonal Antibody Production |
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Fig. 1-1 SDS-PAGE analysis of A Antibody expressed in our mammalian expression system
Lane 1 and 2: reduced; Lane 3 and 4: non-reduced |
Fig. 1-2 IEF analysis of A Antibody expressed in our mammalian expression system |
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2. Recombinant Protein Expression |
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Fig. 2-1 SDS-PAGE analysis of A Protein expressed in our mammalian expression system
Lane 1 and 2: reduced; Lane 3 and 4: non-reduced |
Fig. 2-2 Western Blot analysis of A Protein expressed in our mammalian expression system |
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3. Membrane Protein |
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| Fig. 3 Indirect Immunofluorescence detection of cell membrane protein expressed in our mammalian expression system |
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1. Bacterial Expression: GenScript has developed BacPowerTM technologies to tackle hard-to-express and hard-to-dissolve proteins. Guaranteed package (SC1253): 3 mg purified protein.
2. Yeast Expression: Our yeast expression system employs stable and durable production strains that resemble mammalian protein processing, incorporating features of high yield, high productivity, and dramatic reductions in costs and time requirements.
3. Insect Cell/Baculovirus Expression: GenScript has pioneered methods for the secretion of recombinant proteins from baculovirus-infected insect cells. Our services are suitable for academic institutions and both the pharmaceutical and biotechnology industries.
4. Process Development and Scale-up: GenScript can optimize the system's growth and production media, also providing a thorough analysis of the growth rate, subcultivation ratio, cell mass, and specific and volumetric productivity using productivity-enhancing conditions. Also, a scale-down model can be applied to predict cell performance. GenScript employs a novel and highly productive proprietary system which is featured with predictive high-throughput.
5. Purification: Comprehensive purification method and tag to meet you specific needs. The purification tag can be removed via a specific protease, and the protein is further purified by gel-filtration.
6. Protein characterization: Upon completion of each project, our specialists confirm that the final product meets the client's exact specifications. Typically, this assessment includes any or all of the following: SDS or native PAGE followed by appropriate staining, protein concentration determinations, Western blotting, absorption spectrum, N-terminal sequencing, mass spectrometry, NMR spectroscopy, and activity assays.
7. mAb Sequencing: GenScript's advanced technology offers fast and professional sequencing services for your valuable monoclonal antibodies. Our ISO 9001:2000 certified antibody sequencing service can speed and facilitate your patent applications, database banking, and antibody therapeutics.
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Quotations and Ordering:
 To request a quotation, please fill in the Quotation Form and send it to us by email or fax. Download the Quotation Form in following list:
- For mammalian transient gene expression and protein production service, please download the
 ;
- For mammalian stable R&D cell line development service, please download the
 ;
- For mammalian stable production cell line development service, please download the
 .
To order, please send the completed Quotation Form to us by email or fax with a formal PO (Purchase Order) or credit card information. You can also submit PO/credit card information by phone or via our secure online messaging system.
If submitting samples, please mail them together with a hard copy of the completed Quotation/Order Form to: Bioprocess Services, GenScript, 120 Centennial Ave., Piscataway, NJ 08854, US.
Our customer service representatives are available 24 hours a day, Monday through Friday to assist you.
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