Truncation Library |
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Truncation library allows researchers to determine the minimum length required for epitope activity. The library is generated through a systematic truncation of the peptide's sequence from each terminus. Knowing the positions of key residues via Alanine Scanning Library studies, the truncation fragments can be centered around them.
In many cases, truncation library screening gives knowledge about the peptides with enhanced proteolytic stability. It can act as a tool to investigate peptide drugs undergo metabolic degradation, which is a major inhibiting factor bringing these drugs to the market. |
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Overlapping Peptide
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Alanine Scanning
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Truncation Library
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Positional Scanning
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Random Library
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Scrambled Library
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References
- Svenson J, Stensen W, Brandsdal BO, Haug BE, Monrad J, and Svendsen JS. Antimicrobial peptides with stability toward tryptic degradation. Biochemistry. Mar 2008 25; 47(12): 3777-88.
- Bolger GB, Baillie GS, Li X, Lynch MJ, Herzyk P, Mohamed A, Mitchell LH, McCahill A, Hundsrucker C, Klussmann E, Adams DR, and Houslay MD. Scanning peptide array analyses identify overlapping binding sites for the signalling scaffold proteins, beta-arrestin and RACK1, in cAMP-specific phosphodiesterase PDE4D5. Biochem. J. Aug 2006; 15; 398(1): 23-36.
- Ostermeier M, Nixon AE, Shim JH, and Benkovic SJ. Combinatorial protein engineering by incremental truncation. Proc. Natl. Acad. Sci. U S A. Mar 1999 30; 96(7): 3562-7.
- de Figueiredo P, Roberts RL, and Nester EW. DARTs: A DNA-based in vitro polypeptide display technology. Proteomics. Oct 2004; 4(10): 3128-40.
- Horswill AR, Naumann TA, and Benkovic SJ. Using incremental truncation to create libraries of hybrid enzymes. Methods Enzymol. 2004; 388: 50-60.
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