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Cat. No.
Product Name
Size
Price
Quantity
SD0121 pGS-21a
10 μg
$140.00

pGS-21a


Description
The pGS-21a vector is designed for cloning, high-level expression and convenient purification of proteins fused with both 6xHis and GST. Two 6xHis sequences were introduced into the vector for easy detection and purification of fused proteins. The first 6xHis is fused to GST and the fused tag 6xHis-GST can be cleaved using enterokinase. The second 6xHis can be used for detection and further purification after the cleavage of 6xHis-GST.



Storage
Store at -20°C.


Forward Sequencing Primer
DA0009: T7 (TAATACGACTCACTATAGGG)

Reverse Sequencing Primer
DA0010: T7 Terminator (GCTAGTTATTGCTCAGCGG)

Detailed Map

F1 ori: 1061 - 1516
Polylinker: 834 - 892
Ampicillin: 1648 - 2508

Vector Sequence and Restriction Enzyme Map


Example

  • PGS-21a zoom

  • Document

  • Document-MSDS: 1075_20051003104720.PDF (PDF)
  • Document-DATASHEET: SD0121_datasheet.pdf (pdf)
  • Document-COA: 14126_20111114035314.PDF (PDF)
  • Document-COA: 14127_20111114035314.PDF (PDF)
  • Document-COA: 14128_20111114035315.PDF (PDF)
  • Document-COA: 14129_20111114035316.PDF (PDF)
  • Document-COA: 14130_20111114035317.PDF (PDF)
  • Document-COA: 188619-1_COA.pdf (PDF)
  • Document-COA: 227778-1_COA.pdf (PDF)
  • Document-COA: 227823-1_COA.pdf (PDF)
  • Document-COA: 349747-1_COA.pdf (PDF)

  • References

    • Cervantes S, .et alThe multifunctional autophagy pathway in the human malaria parasite, Plasmodium falciparum.Autophagy. 2014 Jan 1;10(1):80-92.
    • DWD Chung, .et alCharacterization Of The Ubiquitylating Components Of The Human Malaria Parasite'S Protein Degradation Pathway.PLoS One.2012 7(8):e43477.


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