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pRNA-U6.1/Neo

Description
pRNA-U6.1/Neo is a GenScript siRNA expression vector. It is designed for mammalian transfection. It carries a neomycin resistance gene that can be used for establishing stable cell line. It uses a U6 promoter for siRNA expression.



Storage
Store at -20°C


Download Protocol
tm0169


Forward Sequencing Primer
DA0011: pRNA-U6 Forward (TACGATACAAGGCTGTTAGAGAG)

Reverse Sequencing Primer
DA0012: pRNA Reverse (TAGAAGGCACAGTCGAGG)

Detailed Map

Polylinker: 78 - 101
U6 Promoter: 4829 - 77
SV40 Promoter: 850 - 1195
Neomycin: 1236 - 2030
pUC ori: 2744 - 3384
Ampicillin: 3532 - 4392

Vector Sequence and Restriction Enzyme Map



Document

  • Document-PRODUCTINFO: 1107_20051023210728.PDF (PDF)

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