pRNA-U6.1/Zeo

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Description

pRNA-U6.1/Zeo is a GenScript siRNA expression vector. It is designed for mammalian transfection. It carries a zeomycin resistance gene that can be used for establishing stable cell line. It uses U6 promoter for siRNA expression.

Storage

Store at -20°C

Download Protocol

tm0169

Forward Sequencing Primer

DA0011: pRNA-U6 Forward (TACGATACAAGGCTGTTAGAGAG)

Reverse Sequencing Primer

DA0012: pRNA Reverse (TAGAAGGCACAGTCGAGG)

Detailed Map

Polylinker: 628 - 651
U6 Promoter: 301 - 627
SV40 Promoter: 1254 - 1600
Zeomycin: 1688 - 2062
pUC ori: 2732 - 3375
Ampicillin: 3523 - 4383

Vector Sequence and Restriction Enzyme Map

Document

Document-PRODUCTINFO: 1120_20051023214201.PDF (PDF)


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