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*This product is under development*

pRNAT-H1.1/Hygro

Description
pRNAT-H1.1/Hygro is a GenScript siRNA expression vector. It is designed for mammalian transfection. It carries a Hygromycin resistance gene as the selectable marker which can be used for establishing stable cell line. The GFP marker (coral GFP, cGFP) under CMV promoter control can be used to track the transfection efficiency. It uses H1 promoter for siRNA expression.



Storage
Store at -20°C


Download Protocol
tm0169


Forward Sequencing Primer
DA0013: pRNA-H1 Forward (TAATACGACTCACTATAGGG)

Reverse Sequencing Primer
DA0012: pRNA Reverse (TAGAAGGCACAGTCGAGG)

Detailed Map

Polylinker: 6271 - 6294
H1 Promoter: 6171 - 6270
CMV Promoter: 37 - 624
cGFP: 641 - 1360
SV40 Promoter: 2049 - 2394
Hygromycin: 2417 - 3442
pUC ori: 4112 - 4752
Ampicillin: 4900 - 5760

Vector Sequence and Restriction Enzyme Map

Limited Use Label License

The use of CMV promoter is covered under U. S. Patent No. 5,168,062 and 5,385,839 owned and licensed by the University of Iowa Research Foundation and is sold for research use only. Commercial users must obtain a license to these patents directly from the University of Iowa Research Foundation (UIRF), 214 Technology Innovation Center, Iowa City, Iowa 52242. For further information, please contact the Associate Director of UIRF, at 319-335-4546.

Document

  • Document-PRODUCTINFO: 1131_20051023224009.PDF (PDF)

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