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 Name
Size   
Price
 Selected
SD1219
 pRNAT-H1.1/Adeno
10 μg
$350

pRNAT-H1.1/Adeno

Description
GenScript pRNAT-H1.1/Adeno is an adenoviral siRNA shuttle vector. It is compatible with the Stratagene AdEasy Adenoviral Vector System. The vector is designed for transfection in mammalian cells. An H1 promoter is used to drive siRNA expression. The vector contains a cGFP marker under the control of cytomegalovirus (CMV) promoter*, which can be used to track transfection efficiency. The vector also contains a kanamycin resistance gene for transformant selection. LITR and RITR regions are available in this vector for recombinant viral DNA replication.




Storage
-20°C


Download Protocol
tm0165


Forward Sequencing Primer
DA0013: pRNA-H1 Forward (TAATACGACTCACTATAGGG)

Reverse Sequencing Primer
DA0014: pShuttle-H1.1 Reverse (CAAAACTACATAAGACCCCCAC)

Detailed Map

pBR322 origin: 5602 - 6269
Kanamycin: 7059 - 7850
Polylinker: 2112 - 2141
H1 Promoter: 2012 - 2111
CMV Promoter: 1944 - 1356
cGFP: 1333 - 622

Vector Sequence and Restriction Enzyme Map



Document

  • Document-PRODUCTINFO: 1135_20051023224708.PDF (PDF)

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