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You are here: Products » PCR and Cloning » siRNA Expression Vector » siRNA Vector : pRNATin-H1.2/Neo
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Cat. No.
 Name
Size   
Price
 Selected
SD1223
 PRNATin-H1.2/Neo
10 μg
$350

pRNATin-H1.2/Neo

Description
pRNATin-H1.2/Neo is a GenScript inducible siRNA expression vector. The H1.2 promoter is an engineered inducible promoter* containing a tetracycline operator (TetO1). The Tetracycline operator itself has no effect on expression unless the tetracycline repressor (TetR) is present. In the presence of TetR, the promoter effectively binds the TetO1 and blocks the transcription. In the presence of tetracycline or doxycycline, the inducer binds TetR and causes the TetR protein to release the TetO1 site and derepressing the transcription from the H1 promoter.

pRNATin-H1.2/Neo is designed for mammalian transfection. It carries a neomycin resistance gene, which can be used for establishing stable cell lines, and a GFP (coral GFP) marker under CMV promoter** control to track transfection efficiency.



Storage
Store at -20°C


Download Protocol
tm0169


Forward Sequencing Primer
DA0013: pRNA-H1 Forward (TAATACGACTCACTATAGGG)

Reverse Sequencing Primer
DA0012: pRNA Reverse (TAGAAGGCACAGTCGAGG)

Detailed Map

Polylinker: 6122 - 18
H1 Promoter: 6022 - 6121
CMV Promoter: 57 - 644
cGFP: 661 - 1380
SV40 Promoter: 2069 - 2414
Neomycin: 2455 - 3249
pUC ori: 3963 - 4603
Ampicillin: 4751 - 5611

Vector Sequence and Restriction Enzyme Map

Limited Use Label License

The use of CMV promoter is covered under U. S. Patent No. 5,168,062 and 5,385,839 owned and licensed by the University of Iowa Research Foundation and is sold for research use only. Commercial users must obtain a license to these patents directly from the University of Iowa Research Foundation (UIRF), 214 Technology Innovation Center, Iowa City, Iowa 52242. For further information, please contact the Associate Director of UIRF, at 319-335-4546.

Limited Use Label License

This product is covered by United States Patent Applications No. 60/505,677, owned by Genscript Corporation. The purchase of this product conveys to the buyer the limited, non-exclusive, non-transferable right (without the right to resell, repackage, or further sublicense) under these patent rights to perform the siRNA synthesis methods claimed in the patent application for research and development purposes solely in conjunction with this product.

Document

  • Document-PRODUCTINFO: 1139_20051023225522.PDF (PDF)
  • Document-MSDS: 3897_20080213040507.PDF (PDF)

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