pRNAin-H1.2/Shuttle

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pRNAin-H1.2/Shuttle

Description

pRNAin-H1.2/Shuttle is a GenScript adenoviral siRNA shuttle vector. It uses an inducible H1 promoter* for siRNA expression. This promoter contains a tetracycline operator (TetO1). As a competitor, tetracycline or doxycycline can bind this operator to remove the blockade of tetracycline repressor (TetR) from H1 promoter and induce the transcription of siRNA.

pRNAin-H1.2/Shuttle is designed for mammalian transfection. It also contains a neomycin resistance gene under SV40 promoter control for establishing stable cell line.

Storage

-20°C

Download Protocol

tm0165

Forward Sequencing Primer

DA0013: pRNA-H1 Forward (TAATACGACTCACTATAGGG)

Reverse Sequencing Primer

DA0012: pRNA Reverse (TAGAAGGCACAGTCGAGG)

Detailed Map

Polylinker: 4727 - 4750
H1 Promoter: 4627 - 4726
SV40 Promoter: 674 - 1019
Neomycin: 1060 - 1854
pUC ori: 2568 - 3208
Ampicillin: 3356 - 4216

Vector Sequence and Restriction Enzyme Map

Document

Document-PRODUCTINFO: 1151_20051023235958.PDF (PDF)

TECHNICAL MANUAL: 339_20050517003536.PDF (PDF)


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