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 Name
Size   
Price
 Selected
SD1254
 pRNATin-H1.4/Retro
10 μg
$350

pRNATin-H1.4/Retro

Description
An inH1.4 promoter (the same as inH1.2) drives the siRNA expression with the siRNA insert cloned between MluⅠ and XhoⅠsites. pRNA-H1.4/Retro siRNA expression vector contains a hygromycin resistance gene under the control of cytomegalovirus (CMV) promoter* for establishing stable cell line. The vector uses CMV enhancer/promoter joined MSV 5’LTR (CMV/MSV 5'LTR) and MSV 3'LTR for viral transcription and packaging.



Storage
Store at -20°C


Download Protocol
TM0187


Forward Sequencing Primer
DA0025: SD1241 Forward (TGTAGGTTTGGCAAGCTAGC)

Reverse Sequencing Primer
DA0013: pRNA-H1 Forward (TAATACGACTCACTATAGGG)

Detailed Map

5' LTR: 1 - 727
3' LTR: 5460 - 5755
psi+: 757 - 1566
ColE1 ori: 6456 - 7342
SV40 ori & promoter: 6012 - 6356
IRES: 2975 - 3555
Polylinker: 5288 - 5311
H1 Promoter: 5312 - 5411
CMV Promoter: 1604 - 2132
cGFP: 2247 - 2966
Hygromycin: 3593 - 4613
Ampicillin: 7402 - 8262

Vector Sequence and Restriction Enzyme Map



Document

  • Document-PRODUCTINFO: 1274_20051123023059.PDF (PDF)
  • Document-COA: 14132_20111114035318.PDF (PDF)

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