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You are here: Products » PCR and Cloning » siRNA Expression Vector » siRNA Vector : pRNAT-CMV3.1/Neo
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SD1261
 pRNAT-CMV3.1-Neo
10 μg
$350

pRNAT-CMV3.1/Neo

Description
Human cytomegalovirus (CMV) promoter is one of the strongest promoters described. Based on RNA polymerase II system, CMV promoter drives higher-level constitutive expression of genes in a greater variety of mammalian cell lines than RNA-polymerase-III-based promoters such as U6 and H1. In this vector, the CMV promoter drives the expression of siRNA and a SV40 promoter drives the expression of the resistance gene. siRNA cassettes can be easily inserted into the vectors between BamH I and Afl II sites. The siRNA sequence can be confirmed by DNA sequencing using Reverse Sequencing Primer DA0012 (5’-TAGAAGGCACAGTCGAGG-3’). This vector also carries cGFP (coral GFP) for convenient tracking of transfection efficiency.




Storage
Store at -20°C


Download Protocol
tm0172


Forward Sequencing Primer
DA0023: pRNA-CMV Forward (GTACGGTGGGAGGTCTATAT)

Reverse Sequencing Primer
DA0012: pRNA Reverse (TAGAAGGCACAGTCGAGG)

Detailed Map

Polylinker: 584 - 651
CMV Promoter: 1 - 583
cGFP: 1598 - 2317
SV40 Promoter: 1267 - 1598
Neomycin: 2936 - 3730
pUC ori: 4444 - 5084
Ampicillin: 5232 - 6092

Vector Sequence and Restriction Enzyme Map



Document

  • Document-PRODUCTINFO: 1163_20051024021421.PDF (PDF)
  • TECHNICAL MANUAL: 412_20050718094354.PDF (PDF)

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