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 Name
Size   
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 Selected
SD1265
 pRNAT-CMV3.2/Hygro
10 μg
$350

pRNAT-CMV3.2/Hygro


Description
The Human cytomegalovirus (CMV) promoter is one of the strongest promoters described. Based on the RNA polymerase II system, the CMV promoter drives higher-level constitutive expression of genes in a great variety of mammalian cell lines, than RNA-polymerase III-based promoters such as U6 and H1. In this vector, a CMV promoter drives the expression of siRNA, and an SV40 promoter drives the expression of the resistance gene. siRNA cassettes can be easily inserted into the vectors between BamH I and Xho I sites.



Storage
Store at -20°C


Download Protocol
tm0172


Forward Sequencing Primer
DA0023: pRNA-CMV Forward (GTACGGTGGGAGGTCTATAT)

Reverse Sequencing Primer
DA0012: pRNA Reverse (TAGAAGGCACAGTCGAGG)

Detailed Map

Polylinker: 584 - 651
CMV Promoter: 1 - 583
cGFP: 1598 - 2317
SV40 Promoter: 1267 - 1598
Hygromycin: 2911 - 3936
pUC ori: 4636 - 5276
Ampicillin: 5424 - 6284

Vector Sequence and Restriction Enzyme Map



Document

  • Document-PRODUCTINFO: 1240_20051114031700.PDF (PDF)
  • TECHNICAL MANUAL: 412_20050718094354.PDF (PDF)

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