24 hour
You are here: Products » PCR and Cloning » siRNA Expression Vector » siRNA Vector: pRNAT-CMV3.2/Puro
GenScript Share
molecular biology services
Cat. No.
 Name
Size   
Price
 Selected
SD1266
 pRNAT-CMV3.2/Puro
10 μg
$350

pRNAT-CMV3.2/Puro


Description
The human cytomegalovirus (CMV) promoter is one of the strongest promoters described. Based on the RNA polymerase II system, the CMV promoter drives higher-level constitutive expression of genes in a greater variety of mammalian cell lines, than RNA-polymerase III-based promoters such as U6 and H1. In this vector, a CMV promoter drives the expression of siRNA, and an SV40 promoter drives the expression of the resistance gene. siRNA cassettes can be easily inserted into the vectors between BamH I and Xho I sites.



Storage
Store at -20°C


Download Protocol
tm0172


Forward Sequencing Primer
DA0023: pRNA-CMV Forward (GTACGGTGGGAGGTCTATAT)

Reverse Sequencing Primer
DA0012: pRNA Reverse (TAGAAGGCACAGTCGAGG)

Detailed Map

Puromycin: 2911 - 3510
Polylinker: 584 - 651
CMV Promoter: 1 - 583
cGFP: 1598 - 2317
SV40 Promoter: 1267 - 1598
pUC ori: 4210 - 4850
Ampicillin: 4998 - 5858

Vector Sequence and Restriction Enzyme Map



Document

  • Document-PRODUCTINFO: 1242_20051114033755.PDF (PDF)
  • TECHNICAL MANUAL: 412_20050718094354.PDF (PDF)

    • Related Products
    Cat. No.
    		 Name
    Size   
    Price
    		 Selected
    A00185-200
    		 cGFP-tag Antibody, mAb, Mouse
    200 μg(5x40μg)
    $225
    A00185-40
    		 cGFP-tag Antibody, mAb, Mouse
    40 μg
    $50
    1
    		 Gene Synthesis: GenScript's custom gene synthesis service starts as LOW as $0.39/bp, with advanced FREE codon optimization tool and seamless cloning technology.
    2
    		 PCR Cloning and Subcloning: Start GenScript's flexible PCR cloning and subcloning service RIGHT NOW, getting comprehensive packages delivered in 14 business days.
    3
    		 Site-directed Mutagenesis Services: $149/mutation for special mutagenesis bundle. Site-directed Mutagenesis offers point mutations, deletions, and insertions with unparalleled accuracy, unlimited sites, and comprehensive service package.
    4
    		 Plasmid DNA Preparation: GenScript plasmid DNA preparation services offer endotoxin FREE and predominantly supercoiling plasmid DNA from 100 ug to 1000 mg start from $50.