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pRNAT-U6.1/Hygro/siFLuc


Description
pRNAT-U6.1/Hygro/siFluc is a GenScript siRNA expression vector. It is designed for mammalian transfection. The GFP marker (coral GFP, cGFP), which is under CMV promoter control, can be used to track the transfection efficiency. It uses a U6 promoter for siRNA expression. This vector contains an siRNA construct for Firefly luciferase, and can be used as a positive control. It can also be used as a siRNA vector using BamH I and Hind III for siRNA insertion.



Storage
Store at -20°C


Download Protocol
tm0169


Forward Sequencing Primer
DA0011: pRNA-U6 Forward (TACGATACAAGGCTGTTAGAGAG)

Reverse Sequencing Primer
DA0012: pRNA Reverse (TAGAAGGCACAGTCGAGG)

Detailed Map

Polylinker: 78 - 147
U6 Promoter: 6354 - 77
CMV Promoter: 186 - 782
cGFP: 798 - 1517
SV40 Promoter: 2206 - 2551
Hygromycin: 2574 - 3599
pUC ori: 4269 - 4909
Ampicillin: 5057 - 5917

Vector Sequence and Restriction Enzyme Map



Document

  • Document-PRODUCTINFO: 1177_20051024024027.PDF (PDF)

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