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SD1523
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10 μg
$225

pRNATin-H1.2/Neo/siFLuc


Description
pRNATin-H1.2/Neo/siFluc is a GenScript siRNA expression vector. It is designed for mammalian transfection. The GFP marker (coral GFP, cGFP) , which is under CMV promoter control, can be used to track the transfection efficiency. It uses the H1.2 promoter, an engineered inducible promoter containing a tetracycline operator (TetO1), for siRNA expression. This vector contains an siRNA construct for firefly luciferase and can be used as a positive control. It can also be used as an siRNA vector using BamH I and Hind III for siRNA insertion.





Storage
Store at -20°C


Download Protocol
tm0169


Forward Sequencing Primer
DA0013: pRNA-H1 Forward (TAATACGACTCACTATAGGG)

Reverse Sequencing Primer
DA0012: pRNA Reverse (TAGAAGGCACAGTCGAGG)

Detailed Map

Polylinker: 6168 - 64
H1 Promoter: 6068 - 6167
CMV Promoter: 103 - 691
cGFP: 707 - 1426
SV40 Promoter: 2115 - 2460
Neomycin: 2501 - 3295
pUC ori: 4009 - 4649
Ampicillin: 4797 - 5657

Vector Sequence and Restriction Enzyme Map



Document

  • Document-PRODUCTINFO: 1179_20051024024323.PDF (PDF)
  • TECHNICAL MANUAL: 313_20050506154231.PDF (PDF)

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