pRNA-U6.3/Neo/siFLuc

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pRNA-U6.3/Neo/siFLuc

Description

pRNA-U6.3/Neo/siFLuc is a GenScript siRNA expression vector. It is designed for mammalian transfection. It uses U6 promoter for siRNA expression. This vector contains siRNA construct for Firefly luciferase, and can be used as a positive control. It can also be used as a siRNA vector using BamH I and Hind III for siRNA insertion.

Storage

Store at -20°C

Download Protocol

tm0169

Forward Sequencing Primer

DA0011: pRNA-U6 Forward (TACGATACAAGGCTGTTAGAGAG)

Reverse Sequencing Primer

DA0012: pRNA Reverse (TAGAAGGCACAGTCGAGG)

Detailed Map

CMV IE: 4807 - 5290
siFluc: 84 - 141
Polylinker: 78 - 147
U6 Promoter: 5298 - 77
SV40 Promoter: 896 - 1241
Neomycin: 1282 - 2076
pUC ori: 2790 - 3430
Ampicillin: 3578 - 4438

Vector Sequence and Restriction Enzyme Map


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