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pRNA-H1.1/Neo/CTL


Description
pRNA-H1.1/Neo/CTL is a GenScript general-purpose siRNA negative control vector. It is designed for mammalian transfection. It uses an H1 promoter for siRNA expression. The sense sequence shows no homology to any mouse or rat mRNA sequence in the NCBI RefSeq database. It can be used as a negative control and as a siRNA vector. This vector employs BamH I and Hind III for siRNA insertion.



Storage
Store at -20°C


Download Protocol
tm0169


Forward Sequencing Primer
DA0013: pRNA-H1 Forward (TAATACGACTCACTATAGGG)

Reverse Sequencing Primer
DA0012: pRNA Reverse (TAGAAGGCACAGTCGAGG)

Detailed Map

Polylinker: 4700 - 4767
H1 Promoter: 4600 - 4699
SV40 Promoter: 647 - 992
Neomycin: 1033 - 1827
pUC ori: 2541 - 3181
Ampicillin: 3329 - 4189

Vector Sequence and Restriction Enzyme Map



Document

  • Document-PRODUCTINFO: 1215_20051024034858.PDF (PDF)
  • TECHNICAL MANUAL: 313_20050506154231.PDF (PDF)

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