Adenoviral Vector Services
The adenovirus (AV) is a non-enveloped particle containing linear double-stranded DNA (dsDNA). AVs infect both dividing and non-dividing cells and replicate as episomal elements in the nucleus. There exist many AV serotypes. To date, the majority of adenovirus vectors are developed based on serotypes 2 and 5 by replacing either the E1 or E3 gene with a transgene, resulting in the loss of viral replicability. These recombinant viruses are replicable only in cells that express the E1 or E3 gene products at very high concentrations, creating a highly efficient, controllable viral vector system for therapeutic applications.
Service-Related Specifications (SC1166):
- Recombinant AV vector construction: GenScript's adenovirus vectors are standard E1- and E3-deleted AV vectors. Cloning plasmids are available to express the transgene as the single gene product or simultaneously with a GFP reporter under a bi-directional promoter for the purpose of transfection efficiency analysis. An inducible tetracycline adenovirus vector is also available for controlled gene expression.
- Recombinant AV production: Recombinant adenovirus vectors are derived from plaque-forming units (pfu). A single plaque is selected and amplified by serial infection of 293A cells on 20×15 cm plates after transduction with the linearized vector plasmids. The recombinant AVs are purified, titered, and verified by restriction digestion and sequencing.
- Recombinant AV characteristics: Routinely, a single adenovirus vector virus stock is prepared starting from 30×150 mm-cell culture dishes.
- Yield: 1×103 viral particles/cell
- Biological titer: 108-1010 pfu/ml(concentrated)
- Final volume: 1 ml of purified viruses in phosphate buffered saline (PBS)
- Storage: The AV vector batches are best stored at -80°C
Additional Services:
GenScript offers ready-to-use AAV-GFP and AAV-LacZ reporter vectors that can be used as controls. Upon request, GenScript can produce customized AAV vectors pseudotyped with different capsid serotypes.
 For quotation requests, please download and complete GenScript Viral Vector Services Quotation Request Form, and email or fax it to us with a detailed description of project requirements. For questions, please use our secure web server. However, you may also contact us by email at order@genscript.com, phone, or fax.
Orders can be placed by phone, email, or fax with either a formal PO (Purchase Order) or credit card. Please email or fax us a completed GenScript Viral Vector Services Quotation Request Form. Our customer service representatives are available 24 hours Monday through Friday. If target gene is physically available, please submit your target gene along with a hard copy of completed GenScript Viral Vector Services Quotation Request Form to Viral Vector Services, GenScript Corp., 120 Centennial Ave., Suite 105, Piscataway, NJ 08854, US.
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Order by email: order@genscript.com |
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Order by phone: 1-877-436-7274 (Toll-Free) 1-732-885-9188 |
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Order by fax: 1-732-210-0262 1-732-885-5878 |
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