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Yeast Expression System
Yeast fermentation and expression quotation

YeastHIGH™ yeast fermentation and expression services

Yeast fermentation is the most economical eukaryotic expression system for  scale up production of both secreted and intracellular proteins that require post-translational modifications, including humanized antibodies. GenScript's YeastHIGH™ yeast fermentation and expression services utilize a proprietary yeast fermentation technology for high-yield and high quality recombinant protein production in yeast, by selecting stable and durable production yeast strains that resemble mammalian system the most. We are also  equipped with scale-up yeast fermentation capacity up to 500 liters.  In addition, as the largest gene synthesis supplier in the U.S., GenScript deploys free OptimumGene™ codon optimization specific to  yeast strains such as Pichia pastoris and Saccharomyces cerevisiae to further enhance your recombinant protein yields.

Advantages of YeastHIGH™ yeast fermentation and expression services

  • Using proprietary technology: GenScript's YeastHIGH™ technology takes consideration of all essential parameters for high-level recombinant protein production in yeast fermentation, including promoters, optimal vector copies  per cell, inducibility, signal peptides and cellular location etc., ensuring high yield and high quality protein production in yeast cells
  • Large-scale protein production capacity: GenScript is not only equipped with yeast fermentation capacity up to 500L, but also has large-scale filtration system to enhance recombinant protein purity and yields
  • Streamlined services: GenScript can take your project directly from gene synthesis, to recombinant protein expression, as well as downstream application development, such as  antibody production and assay development

Package details of YeastHIGH™ yeast expression and purification services (SC1257)

Customer provides Steps Description Timeline# Deliverables Price
Target sequence
(DNA or protein)/DNA
template/expression construct
Gene synthesis & Subcloning (optional)
  • Subcloning into appropriate expression vector
  • Plasmid maxi preparation
  • Construct linearization
2 weeks
Construct report
Starting at $2,900

Yeast fermentation and expression quotation
Expression & evaluation
  • Transformation of linear construct into appropriate host
  • Confirmation of positive transformants via PCR
  • Expression confirmation
  • SDS-PAGE gel and/or Western blot
2 weeks
Expression data
1L expression & purification
  • 1 L yeast culture expression
  • One-step purification, or additional purification to reach ≥90% purity
  • QC* by SDS-PAGE, Western blot and MALDI-TOF
2 weeks
  • Purified proteins
  • QC report
Large Scale protein production & purification (optional)
  • Large-scale production up to 2,000 L (Employs fermenters and TFF large-scale filtration system)
  • Deliverables available at ≥90% purity or higher
  • Tag removal
2-3 weeks
  • Yeast paste/purified proteins
  • QC data

*QC: SDS-PAGE will be performed to determine protein purity. BCA or Bradford assay will be used to measure protein concentration and Western blot using anti-His or anti-GST antibodies will be performed, to determine protein identity. MALDI-TOF peptide mapping will be used in addition to Western Blot, to determine protein identity (if necessary)

If you are looking exclusively for yeast fermentation services without downstream protein purification option please choose from our flexible scale yeast fermentation options below.

Package details of YeastHIGH™ yeast fermentation services

Customer provides
(any one)
Fermentation Scale
Timeline#
Deliverables
Price
  • Target sequence
  • DNA/Expression construct
  • Glycerol stock
  • Microbial growth plate
5L
1-2 weeks
  • Cell paste
  • Batch records
  • QC datasheet

Regular fermentation
starting from $480

Bacterial fermentation services quotation

(using downloadable inquiry form)
High cell density fermentation
starting from $765

Bacterial fermentation services quotation

(using downloadable
inquiry form)
10L
20L
50L
100L
2-3 weeks
200L
300L
500L
3-4 weeks

#All timelines are approximate

Besides yeast fermentation services, GenScript also offers recombinant protein production in bacterial, insect and mammalian expression systems to meet different protein production needs.

Yeast fermentation and expression quotation

Quotations and Ordering

Yeast fermentation and expression quotations & ordering