GenScript provides the synthesis service for RT-qPCR primers and probes of the sequences recommended by WHO and CDC.
| Gene Name | Oligo Name | oligo Sequence(5'-3') | Length (nt) |
|---|---|---|---|
| RdRP gene | RdRP_SARSr-F2 | GTGARATGGTCATGTGTGGCGG | 22 |
| RdRP_SARSr-R1 | CARATGTTAAASACACTATTAGCATA | 26 | |
| RdRP_SARSr-P2 | CAGGTGGAACCTCATCAGGAGATGC | 25 | |
| RdRP_SARSr-P1 | CCAGGTGGWACRTCATCMGGTGATGC | 26 | |
| E gene | E_Sarbeco_F1 | ACAGGTACGTTAATAGTTAATAGCGT | 26 |
| E_Sarbeco_R2 | ATATTGCAGCAGTACGCACACA | 22 | |
| E_Sarbeco_P1 | ACACTAGCCATCCTTACTGCGCTTCG | 26 | |
| N gene | N gene-F | GGGGAACTTCTCCTGCTAGAAT | 22 |
| N gene-R | CAGACATTTTGCTCTCAAGCTG | 22 | |
| N gene-P | TTGCTGCTGCTTGACAGATT | 20 | |
| ORF1ab gene | ORF1ab-F | CCCTGTGGGTTTTACACTTAA | 21 |
| ORF1ab-R | ACGATTGTGCATCAGCTGA | 19 | |
| ORF1ab-P | CCGTCTGCGGTATGTGGAAAGGTTATGG | 28 |
We have two different standards to fulfill your request:
GenScript also offers any other customized primers and probes for COVID-19 research. Please contact our technical support managers for more information via email [email protected]. Our technical account managers will contact you in 24 hours.
What you need to know about SARS-CoV-2 qRT-PCR detection
assay
Forbes article on Synthetic Bio Companies Racing to Fight Coronavirus here
Reuters article: Countries rush to build diagnostic capacity as coronavirus spreads
Genetic Engineering & Biotechnology News: Detecting Coronavirus Cases as Outbreak Grows here
GenScript is mentioned in a Coronavirus story mostly about Novacyt that was written by Reuters, but picked up by The New York Times here today
Medical Device & Diagnostics Online article here
LabPulse article here
GenomeWeb article here
Biospace Blog: China Coronavirus Update: Continued Spread, Run on Antivirals and Facemasks, Baby Diagnosed
To place order or need technical support for coronavirus detection assay, please contact:
The plasmids containing parts of SARS-CoV-2 RdRP gene, N gene, E gene that can be used as positive controls for SARS-CoV-2 detection are in stock now. A group of plasmids carrying the encoding sequences of surface glycoprotein and nucleocapsid phosphoprotein are also available. The quick online order channel has been launched on MolecularCloud.
