GenScript develops fast and reliable SARS-CoV-2 diagnostic assays for both research and clinical use to help protect public health.
GenScript's one-step RT-qPCR detection assays target ORF1ab gene, RdRP gene, N gene and E gene in SARS-CoV-2 genome (GenBank sequences NC_045512.2). The fast and reliable nucleic acid detection method can help researchers better understand the biology of COVID-19 and guide clinical diagnostics and treatment.
SARS-CoV-2 RT-qPCR Detection Assay
Key Features
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Complete assay set
High-throughput
Super sensitive
No background noise
- Full reagent set with primers, probes, reagents and positive control plasmids.
- Up to 3-plex TaqMan™ RT-qPCR assay to have high-throughput and high specificity.
- High sensitivity and ultra-low noise.
- Detection kit with CE markings.
- RUO or clinical used(ISO 13485) primers and probes.
- Each assay's design is impacted by the accuracy of the publicly SARS-CoV-2 genome sequence.
- Each assay's performance is impacted by a range of uncontrolled and un-tested factors such as sample quality, various sample extraction methods, and data analysis variation.
- Shelf-life and storage condition of the kit is based on stability tests including 2-8 ℃ stability test, freeze-and-thaw test, and 37 ℃ accelerated stability test. Long-term stability test is still on-going.
GenScript provides the synthesis service for RT-qPCR primers and probes of the sequences recommended by WHO and CDC.
| Gene Name | Oligo Name | oligo Sequence(5'-3') | Length (nt) |
|---|---|---|---|
| RdRP gene | RdRP_SARSr-F2 | GTGARATGGTCATGTGTGGCGG | 22 |
| RdRP_SARSr-R1 | CARATGTTAAASACACTATTAGCATA | 26 | |
| RdRP_SARSr-P2 | CAGGTGGAACCTCATCAGGAGATGC | 25 | |
| RdRP_SARSr-P1 | CCAGGTGGWACRTCATCMGGTGATGC | 26 | |
| E gene | E_Sarbeco_F1 | ACAGGTACGTTAATAGTTAATAGCGT | 26 |
| E_Sarbeco_R2 | ATATTGCAGCAGTACGCACACA | 22 | |
| E_Sarbeco_P1 | ACACTAGCCATCCTTACTGCGCTTCG | 26 | |
| N gene | N gene-F | GGGGAACTTCTCCTGCTAGAAT | 22 |
| N gene-R | CAGACATTTTGCTCTCAAGCTG | 22 | |
| N gene-P | TTGCTGCTGCTTGACAGATT | 20 | |
| ORF1ab gene | ORF1ab-F | CCCTGTGGGTTTTACACTTAA | 21 |
| ORF1ab-R | ACGATTGTGCATCAGCTGA | 19 | |
| ORF1ab-P | CCGTCTGCGGTATGTGGAAAGGTTATGG | 28 |
Order information for the above primers and probes or any customized primers and probes:
We have two different standards to fulfill your request:
- Research use only
- Clinical used (ISO13485 certified synthesis)
GenScript also offers any other customized primers and probes for COVID-19 research. Please contact our technical support managers for more information via email [email protected]. Our technical account managers will contact you in 24 hours.
GenScript Press Release about COVID-19
- Forbes article on Synthetic Bio Companies Racing to Fight Coronavirus here
- Reuters article: Countries rush to build diagnostic capacity as coronavirus spreads
- Genetic Engineering & Biotechnology News: Detecting Coronavirus Cases as Outbreak Grows here
- GenScript is mentioned in a Coronavirus story mostly about Novacyt that was written by Reuters, but picked up by The New York Times here today
- Medical Device & Diagnostics Online article here
- LabPulse article here
- GenomeWeb article here
- Biospace Blog: China Coronavirus Update: Continued Spread, Run on Antivirals and Facemasks, Baby Diagnosed
To place order or need technical support for coronavirus detection assay, please contact:
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Email: [email protected]
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Phone: 1-877-436-7274 (Toll-Free);1-732-885-9188
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Fax: 1-732-210-0262
Plasmids Used for SARS-CoV-2 Detection and Research are In Stock Now!
The plasmids containing parts of SARS-CoV-2 RdRP gene, N gene, E gene that can be used as positive controls for SARS-CoV-2 detection are in stock now. A group of plasmids carrying the encoding sequences of surface glycoprotein and nucleocapsid phosphoprotein are also available. The quick online order channel has been launched on MolecularCloud.
