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GenCRISPR™ Mammalian Cell Line Services

CRISPR Gene Editing Services

CRISPR is revolutionizing the field of gene editing. It has the potential to alter the course of research and drug discovery, by providing scientists with a powerful tool to change any gene, in any cell in a highly targeted manner and without introducing foreign DNA. The benefits of CRISPR/Cas9 over previous forms of gene editing, such as TALENs and zinc finger nuclease (ZFN), are that it is much simpler to implement and has higher efficiency at performing bi-allelic gene modifications.

GenScript is pleased to introduce GenCRISPR™, a full CRISPR-based gene editing service, to produce a genetically modified cell using any mammalian cell line and targeting any gene.  Our scientists are experts at performing gene editing with CRISPR, from designing gRNA constructs for CRISPR to transfection and single clone generation of a wide range of cells, including difficult-to-transfect and tumor cell lines.

GenCRISPR™ cell line service Cell Lines Timeline Deliverables and Details Price

EZ Knock-out cell line service
(SC1755)

  • Single gene editing KO cell line
  • Customer specifies gene/locus region and cell line of interest*
  • Transfection-based
  • Validated gRNA-Cas9 constructs
30 easy-to-handle cell lines 13-16 weeks
  • 2 full-allelic knock-out cell lines validated by sequencing
  • 2 cryovials of cells per each clone, mycoplasma-free and 90% viability.
  • 1 negative control cell line*
  • Biweekly update of project progress

***Validation of full-allelic knock-out cell lines at mRNA or protein level upon request

100 medium difficulty cell lines 13-16 weeks

Customized Knock-out cell line service
(SC1652)

  • Single or multiplex genes editing KO cell line
  • Customer specifies gene/locus region and cell line of interest**
  • Transfection based
  • Validated gRNA-Cas9 constructs
  • Cas9 stable cell pool service available for difficult cell lines (SC 1758)
Any cancer or immortalized cell line of interest

16-20 weeks
(easy-to-handle lines)

20-26 weeks
(difficult-to-handle lines)

  • *1-2 full-allelic knock-out cell line(s) validated by sequencing
  • 2 cryovials of cells per each clone, mycoplasma-free and 90% viability.
  • 1 negative control cell line*
  • Biweekly update of project progress

***Validation of full-allelic knock-out cell lines at mRNA or protein level upon request

Lenti-CRISPR KO Service
(SC1652-V)

  • Single gene editing KO cell line
  • Customer specifies gene/locus region and cell line of interest**
  • Lentiviral based for difficult-to-transfect cell lines.
  • Cas9 stable cell pool service available for difficult cell lines (SC 1758)
Any cancer or immortalized cell line of interest 16-26 Weeks
  • 1 full-allelic knock-out cell line(s) validated by sequencing
  • 2 cryovials of cells per each clone, mycoplasma-free and 90% viability
  • Cas9-stable cell pool as negative control
  • Biweekly update of project progress

***Validation of full-allelic knock-out cell lines at mRNA or protein level upon request

*It is preferred that customers provide their own cell lines, but cell lines can also be purchased by GenScript for an additional fee.

**Prices may vary based on project specifications

***Validation services for knock-out cell lines can be found under the "Add On Services" tab.

Quotations and Ordering

For quotation requests:

To place an order:

Our customer service representatives are available 24 hours a day, Monday through Friday, to assist you. However, you may also contact us by email, phone, or fax.

 
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