Overview

CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats. CRISPR sequences were originally identified in the Escherichia coli (E. coli) genome, and were found to function as part of an RNA-based adaptive immune system to target and destroy genetic parasites at the DNA level. CRISPR-associated protein (Cas) is an endonuclease that cuts foreign DNA, allowing integration into the host genome. Cleavage only occurs when there is a protospacer adjacent motif (PAM) around the targeted sequence of the invading DNA, ensuring highly accurate targeting. Researchers studying CRISPR have adapted it for use as a tool for genetic modification of the target host genome. CRISPR/Cas9 has recently become a popular genome editing tool, due to its simplicity and versatility.

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Workflow

CRISPR gene editing workflow

Service Specifications

Service Name Service Detail Cell Line Options Deliverables Delivery Time
GenCRISPR™
EZ knockout Service
  • Single gene knock out cell pool/cell line
  • Multiplexed gene knock out cell pool/cell line
Available for 90+ popular transfection-suitable cell lines, including A549, CHO-K1, HEK293, HEK293T, HT-29, MDA-MB-231, 4T1, A20, HCT116, MCF7, MDCK, U937, RPMI 8866, etc.
  • One knockout cell pool/two full-allelic knockout cell lines validated by sequencing
  • One negative control cell pool transfected with non-targeting gRNA
  • Biweekly project updates
3-6 weeks (cell pool)
9-15 weeks (cell clone)
GenCRISPR™ Customized Knockout Service 8-11 weeks (cell pool)
16-24 weeks (cell clone)
GenCRISPR™ Customized Knock-In Service
  • Point mutation
  • Seamless point mutation
  • Endogenous locus/gene tagging/reporter
  • Cell model for genetic variation study (including SNV, SV, InDel, etc.)
  • Safe harbor insertion of GOI
Any cell line
  • 1-2 knock in cell lines validated by sequencing
  • One negative control cell pool transfected with non-targeting gRNA
  • Biweekly project updates
12-25 weeks (Cell clone)
GenCRISPR™ Whole Gene Deletion Service
  • Whole gene deletion
Any cell line
  • One deletion cell pool/full-allelic deletion cell line validated by sequencing
  • One negative control cell pool transfected with non-targeting gRNA
  • Biweekly project updates
13-23 weeks (cell clone)

It is preferred that customers provide their own cell lines, despite available cell lines can be purchased by GenScript for an additional fee. Please note that we currently do not provide genome editing service for primary cells, stem cells or iPS cells.
Validation services for CRISPR cell lines can be found under the "Add On Services" tab.

Add-On Service

Add-On Service Description
Reverse transcription (RT)-PCR Validate the positive cell pool/clones at mRNA level
FACS analysis Validate the transgene expression by FACS analysis. A validated antibody with specific binding to target protein or reporter tag must be provided.
Western Blot Validate the positive clones by western blot. A validated antibody in the host cells with specific binding to target protein must be provided.
Expression stability test Validate the GOI expression stability by WB or FACS in 15/20 generation.
One additional clone QC and deliver one additional positive clone.
Off-target analysis Characterize one knockout clone by sequencing the top 5 of potential off-target sites. 

Case Studies

Resources

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CRISPR Handbook

Enabling Genome Editing and Transforming Life Science Research

Free Download
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CRISPR Knock-in Comprehensive Guide

Step-by-step instructions for your CRISPR knock-in experiments.

Free Download

Get in Touch
with GenScript Custom
CRISPR Experts

Get in Touch with GenScript Custom CRISPR Experts
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