CRISPR Genome Editing in Bacteria
GenScript is pleased to introduce the microbial genome editing service for knock-in, knock-out or gene replacement in bacteria (E. coli). Our service employs a two vector system to target up to three genes simultaneously, ensuring editing accuracy down to the base pair. In addition, to further improve efficacy in bacteria, our scientists have developed a novel λ Red-CRISPR/Cas9 system, which leverages the efficiency of traditional λ Red recombineering with the ease of CRISPR/Cas for seamless, targeted genome editing.
"GenScript's CRISPR service was a life saver for my research. We were totally stuck on a specific point mutation in our E.coli strain, and GenScript was able to step in and get it done. Updates are provided through every step of the process and they've been a pleasure to deal with."
- Chad Johnston, Ph.D., Banting Postdoctoral Fellow in the Collins Lab at MIT.
Advantages of GenScript’s CRISPR microbial gene editing system
CRISPR/Cas technology has quickly become one of the easiest and most effective genome editing systems, and when combined with λ Red recombineering has several advantages over the most common editing strategies:
- Seamless
- Multigene editing: can knock-out up to 3 genes simultaneously
- Precision down to the base pair
- Easy selection: no selectable marker is required
Service Details
| Service | E. coli Knock-out | E. coli Knock-in |
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| Guide RNA Design | Our expert scientists will optimize your guide RNA sequence for maximized efficiency and minimal off-target effects using design algorithms created at the Broad Institute of MIT and Harvard. |
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| Materials Needed from Clients |
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| Multiplex Editing | Up to 3 targets | Single knock-in/replacement |
| Deliverables | Engineered strain in glycerol stock | |
| Quality Control |
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| Delivery Time | Starting from 4 weeks | |
| Pricing | Starting from $4,000 | Starting from $5,400* |
*Pricing does not include the additional cost for gene synthesis, if required.
Quotations and Ordering
For quotation requests:
Complete our online request form and our service representative will get back to you with a free quote within 1 business day.
To order:
- Orders can be placed by phone, email, or fax with a formal PO (Purchase Order) or credit card.
Our customer service representatives are available 24 hours a day, Monday through Friday, to assist you.
Acknowledgements
We would like to acknowledge Professor Sheng Yang (Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences) for contributing the original plasmids for the microbial genome editing service. The plasmids were subsequently engineered by the GenScript team to meet the service needs.
