GANP® Transgenic Mice
GenScript has licensed a unique GANP® technology from Transgenic Inc for the production of high-affinity and high-specificity antibodies. These mice can be chosen when high affinity and high specificity antibodies are needed for certain applications. GANP mice are available for all our hybridoma services including our Fast Monoclonal Antibody Services, Phospho-Specific mAb Service, Customized Monoclonal Antibody Packages, and Antibody Drug Development Services.
- High affinity: GANP® mouse produces mAbs with extraordinarily high affinity.
- High specificity: GANP® mouse antibodies can be used to establish a highly-specific ELISA system that distinguishes similar substances.
GANP, the acronym for Germinal Center Associated Nuclear Protein (GANP), is a recently discovered protein, over-expressed in B-cells centered in embryo. It can be up-regulated in germinal center (GC) B cells upon immunization with T-cell dependent antigen. GANP carries two domains: a domain that generates RNA primers under the regulation of CD40/CD40L interaction (RNA-primase domain) and another domain of the MCM3-binding activity. GANP can augment the induction of somatic hypermutation (SHM) in the V regions, resulting in the affinity maturation of V regions during the proliferation and differentiation of Ag-driven B cells in GCs. Accumulating research has suggested that GANP is important in the reconstruction of antibody genes, and transgenic mice expressing high level of GANP can be used to produce antibodies with high affinity and specificity.
Nature Communications 4, Article number:1830 | doi:10.1038/ncomms2823 | Received 15 October 2012 | Accepted 28 March 2013 | Published 07 May 2013
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J Biol Chem. 2009 Oct 16;284(42):29041-9.
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GANP® mice produce monoclonal antibodies with higher affinities than BALB/c mouse-produced antibodies
Affinity ranking and affinity measurements taken by BIAcore for the monoclonal antibodies generated from GANP® mice or BALB/c mice.
Guideline for GANP® transgenic mouse offering
GenScript has signed a license agreement with TransGenic Inc., and is authorized to use GANP® transgenic mice to provide custom antibody service worldwide (excluding Japan). According to the agreement, GenScript needs to report to Transgenic Inc. the names of customers who have used GANP® services through GenScript.
Any customer using GANP® mice through GenScript Services for research use only does not need to pay an additional fee to Transgenic Inc. However, Transgenic Inc. may charge additional license fees and/or royalties if the antibody becomes a commercial product. GenScript does not have the right to grant the commercial sublicense to any third party. The clients must negotiate with TransGenic Inc. to obtain their own commercial license though GenScript is currently working on obtaining this ability to streamline our customers' experience.
Quotation and Ordering
Quotation and Ordering
To request a quotation, please download and complete our Quick Quotation Request Quick Quotation Request
To submit an antigen, mail it together with a completed hard copy of Antigen Submission Antigen Submission Form
Form to Antibody Services, GenScript, 860 Centennial Avenue., Piscataway, NJ 08854, US.
Please email or fax the quotation request and the antigen submission form to GenScript.
Our customer service representatives are available 24 hours a day, Monday through Friday to assist you.