Overview

GenEZ™ ORF clones offer the highest flexibility in ORF clone customization. Choose from our standard mammalian expression-ready cloning vector or over 150 popular commercial cloning vectors for custom cloning.

Standard Expression Cloning Vector

GenScript's GenEZ™ ORF clones are seamlessly cloned into our standard mammalian expression cloning vector, pcDNA3.1+/C-(K)-DYK, using our GenBuilder™ seamless cloning technology. Driven by a CMV promoter, pcDNA3.1+/C-(K)-DYK is equipped with a C-terminal DYKDDDDK tag for easy protein detection and purification. GenEZ™ ORF clones are delivered expression and transfection ready, saving researchers valuable time in the lab. There is no additional charge for cloning into pcDNA3.1+/C-(K)-DYK.

You can see more detailed vector information & maps.

Our standard vector pcDNA3.1+/C-(K)-DYK is an expression/transfection ready cloning vector for mammalian systems

Expression System Promoter Bacterial Selection Copy number Epitope tag Length
Mammalian CMV Ampicillin High C-DYK 5444

Custom Cloning Vectors

For enhanced flexibility, GenEZ™ ORF clones can be directly custom cloned into one of over 150 expression vectors that we offer compatible with mammalian or bacterial systems including pLenti, Gateway pDONR, pcDNA, pET, pPIC, and pUC vectors series.

In addition, for GenEZ™ ORFs cloned into custom vectors can be further customized by the addition of up to 30 bp of 5' or 3' flanking sequence – free of charge.

ORF Cloning Vectors Maps*

(Click any vector name to download the vector map in PDF)

Mammalian Yeast Baculovirus/Insect Bacterial
pcDNA3.1(+)
pcDNA3.1(-)
pcDNA3.1(+)_myc-His A
pcDNA3.1(+)_myc-His B
pcDNA3.1(+)_myc-His C
pcDNA3.1(-)_myc-His A
pcDNA3.1(-)_myc-His B
pcDNA3.1(-)_myc-His C
pCI-Neo
pcDNA3.1+C-DYK
pcDNA3.1+C-HA
pcDNA3.1+C-6His
pcDNA3.1+C-Myc
pcDNA3.1+N-DYK
pcDNA3.1+N-HA
pcDNA3.1+N-6His
pcDNA3.1+N-Myc
pcDNA3.1+N-GST(Thrombin)
pcDNA3.1+N-GST(TEV)
pcDNA3.1/Hygro(+)
pcDNA3.1/Hygro(-)
pcDNA3.1/Zeo (+)
pcDNA3.1/Zeo (-)
pCMV-3Tag-1a
pCMV-3Tag-2a
pCMV-3Tag-3a
pCMV-3Tag-4a
pcDNA3.1+C-eGFP
pcDNA3.1+N-eGFP
pcDNA3.1+P2A-eGFP
pcDNA3.1+P2A
pcDNA3.1+N-DYK-P2A
pcDNA3.1+C-DYK-P2A
pCMV-3Tag-1a-P2A
pCMV-3Tag-3a-P2A
pcDNA3.4
pGenLenti
pAO815
pPIC 3.5k
pPIC9
pPIC9K
pPICZA
pPICZB
pPICZC
pPICZalphaA
pPICZalphaB
pPICZalphaC
pESC-TRP
pESC-URA
pESC-HIS
pESC-LEU
pBacPAK8
pBacPAK9
pAcG2T
pAcHLT A
pAcHLT B
pAcHLT C
pAcGHLT A
pAcGHLT B
pAcGHLT C
pAcSG2
pBAC-1
pFastBac1
pFastBacHT-A
pFastBacHT-B
pFastBacHT-C
pFastBac-Dual

*Not all vectors maps shown here. Vectors not shown may need to be shipped by the customer to GenScript for ORF custom cloning.

Search Your Latest Version ORFs at GenScript Now

You can enter the following as search terms:

  • Entrez Gene ID (e.g. 7157)
  • gene symbol (e.g. TP53)
  • gene name (e.g. tumor protein p53)
  • gene synonyms (e.g. FLJ92943)
  • Ensembl ID (e.g. ENSG0000141510)
  • RefSeq Accession (e.g. NM_000546)
  • Species can be input after the keyword, using format "keyword [species:$species]" where $species can be name of species (like human or rat) or taxon id (like 9606).

Where to target the version number? Example

GenScript

GenScript
GenScript

Get more search, click here.

REQUEST A QUOTE

feedback

Do you like the current new website?

Hate

Dislike

Neutral

Like

Love

*