Step 1: Design your Gene 1. Sequence information send
* Gene name (for your records only):
5' sequence:
* Sequence: DNA    Protein
Batch Orders (.xls)
If your sequence length is less than 8,000 bp, please submit the request through our Gene Synthesis ordering system.
3' sequence:
Search Enzyme

2. Codon Optimization
Yes
No

3. Cloning
Standard Cloning
Custom Cloning
Other Requirements:

4. Plasmid Prep
Standard delivery: 4 μg (Free of charge)
Custom plasmid preparation (additional charge) New! 20% OFF (ends March 31st, 2017)

5. Mutagenesis
Mutagenesis 1

The optional Search Enzyme tool makes it easy to add restriction sites to flank your sequence.

Codon Optimization can improve protein expression levels and reduce gene sequence complexity. Optimization using GenScript’s patented OptimumGene algorithm is a complimentary option for all gene synthesis orders.

Turnaround times listed below are for genes with standard sequences. Complex sequences may require additional time to synthesize.
 Sequence length  Standard Service  Fast Service  Rush Service
≤ 800 bp
8 - 10 days
7 days
4 days
801 bp - 1,200 bp
8 - 10 days
7 days
5 days
1,201 bp – 1,500 bp
8 - 10 days
9 days
7 days
1,501 bp – 2,000 bp
12 - 14 days
9 days
7 days
2,001 bp - 3,000 bp
12 - 14 days
11 days
 
3,001 bp - 4,500 bp
16 - 20 days
15 days
 
4,501 bp - 5,000 bp
21 - 25 days
15 days
 
5,001 bp - 6,000 bp
21 - 25 days
   
6,001 bp - 8,000 bp
23 - 30 days
   

Standard Cloning: choose from 6 pUC vectors. No additional cost or turnaround time. Available with any gene synthesis service (Standard, Rush, or Fast).
Express Cloning: choose from 66 Express Cloning vectors. Add 2 days turnaround time. Available with Standard or Fast gene synthesis.
Custom Cloning: any vector of your choice (mail in an aliquot). Add 5 days turnaround time. Available with Standard or Fast gene synthesis.
If you require cloning for Rush gene synthesis, please make a note in the "Other Cloning Requirements" box below. Turnaround timed guarantee for Rush genes do not apply if cloning is requested. An estimated delivery date will be provided to you by email within 24 hours.

Select desired quantity, quality, and QC options for your plasmid DNA.

100 μg transfection grade plasmid DNA. Starting from $50.

For vectors that appear in the drop-down menu, you do not need to provide any information or materials. For any other vector, please provide the requested information; after your order is placed, we will provide you a shipping label to mail us an aliquot of the vector.

Standard Cloning into pUC57-Brick vectors is free of charge. Custom Cloning into any other vector of your choice is available; additional charges apply.

100 μg transfection grade plasmid DNA. Starting from $50.

Only test for E.coli

 QC Items Research Grade Transfection Grade Specification
Appearance Clear, no visible particles
A260/280 1.8-2.0
Supercoiled content Based on the grade
Residual RNA Not detectable on agarose gel
Genomic DNA Not detectable on agarose gel
Concentrations 0.5-4 mg/mL upon request
Restriction Analysis Conforms to reference
Endotoxin Assay (LAL test)- Based on the grade
Bio-burden AssayOptional No growth on agar plate after 48 hours
For most sequences, a quote will be instantly emailed to you stating our guaranteed turnaround time and price. For complex requests, our Ph.D.-level Technical Account Managers will email you a quote within one business day. 

For Batch Orders: You may fill out and upload the Quick Quotation Request Form, or send batch gene sequences in FASTA format to gene@genscript.com.

For assistance in requesting a quote or placing an order for gene synthesis, email gene@genscript.com, or call at 1-877-436-7274 (Toll-Free). Our experienced, Ph.D-level representatives will help manage your projects from sequence design, optimization, synthesis, cloning and beyond without any additional charges (Certain Terms and Conditions may apply).