guide RNA constructs

Search our gRNA database to find validated gRNA sequences targeting any human or mouse gene.  This is the fastest way to get your expression-ready gRNA constructs!

If you need custom gRNA sequence design (e.g. for knock-in, for targeting non-coding genomic regions, or for species other than mouse or human), please use the form below.  

Gene target information:
gRNA for CRISPR genome editing with WT SpCas9
Empty gRNA vectors (no gRNA)
gRNA for CRISPR genome editing with SpCas9D10A – Nickase
gRNA for CRISPR genome editing S. aureus Cas9 (SaCas9)
gRNA for transcription activation with SAM
1. gRNA name & sequence
*gRNA name:
*Species:
Synthesize my specified gRNA sequence
Design and synthesize gRNA sequences for my target sequence
*
*



2. Vector
3. Plasmid delivery
Standard delivery: 4 μg Research Grade (Free of charge)
Custom plasmid preparation (additional charge)


You may enter a DNA sequence in FASTA format, or you may enter the name of the gene you wish you target.

We recommend using 2 or more unique gRNA for each gene you wish to target, in order to increase the likelihood of successful genome editing without off-target effects.

100 μg transfection grade plasmid DNA. Starting from $50.

10µg free upgrade transfection grade plasmid
How to store your vector? Please contact our customer service representatives or email support@genscript.com.
 QC Items Research Grade Transfection Grade Specification
Appearance Clear, no visible particles
A260/280 1.8-2.0
Supercoiled content Based on the grade
Residual RNA Not detectable on agarose gel
Genomic DNA Not detectable on agarose gel
Concentrations 0.5-4 mg/mL upon request
Restriction Analysis Conforms to reference
Endotoxin Assay (LAL test)- Based on the grade
Bio-burden AssayOptional No growth on agar plate after 48 hours
If you have special QC requirements such as sterile filtering, please email gene@genscript after submitting your quote request.