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How many proteins do you want to target?
I want to target
If you are not sure which package to choose, please send an email to firstname.lastname@example.org. Our PhD-level technical account managers will contact you within 24h to discuss your project details and identify the best package choices for you.
GenScript employs the use of sophisticated algorithms making it possible to predict which antigen sequences represent regions of protein which are exposed in the native folding confirmation and will be highly antigenic, increasing the success of generating effective antibodies.
The host of your target antigen is the type of organism that the protein or peptide sequence to be used as an antigen for antibody generation originally came from. *This is not necessarily the same as the host species which will be used to produce your antibody, which is rabbit for all PolyExpress™ packages.
An accession number is a unique identification given to a biological polymer sequence and may be found in protein databases such as UniProt.
Peptides created through GenScript's OptimumAntigen™ design program have many advantages over full proteins when it comes to antibody production. Our OptimumAntigen™ Design Tool combines the industry's most advanced algorithms with GenScript's time-tested expertise. Each peptide antigen is measured against several protein databases to confirm the desired antibody and epitope specificity. We guarantee a polyclonal antibody with ELISA titer of 1:32,000 or better for any host with the aid of our antigen design tool and unique antibody production technology, or we will restart the project free of charge until the guarantee is met. Why are peptide antigens better? It is thought that anti-peptide antibodies against carefully selected peptides are more specific than those generated from longer sequences, such as native protein. With antibodies against whole protein sequences, it is impossible to know whether the epitopes are located within the folded protein while anti-peptide generation gives some control over this factor and helps to identify surface epitopes.
Our analysis shows that there is a ≥ 94% success rate in recognizing native proteins using WB with PolyExpress™ Gold (3 different regions of the antigen being used as targets for antibody generation) and ≥84% success with PolyExpress™ Silver (2 different regions being used for antibody generation). These rates apply when GenScript-deigned peptide antigens are generated. With 3 different regions of the antigen being used targets for antibody generation, there is a much greater chance that you will produce an antibody which recognizes your native protein. Ordering PolyExpress™ Gold and generating 3 antibodies at one time will save you time as you will not have to test the first antibody before determining that you should target another part of the antigen and try again, while optimizing your outcome as ordering more antibodies will not increase the already high ≥ 94% success rate much.
Lyophilized delivery means that the antibody has been freeze-dried for shipment and storage. The process of lyophilization extends the shelf life and preserves the bioactivity of antibodies. Unlabeled antibodies are stable in this form at room temperature, without loss of quality, for weeks or even months and for years at 4°.
Antibodies delivered in liquid form have been reconstituted and so the antibody is ready for use. This antibody is generally stable at -20° for one year. Delivery of antibodies in liquid form protects them from possible loss of function which may be associated with lyophilization in some cases, more often involving mAbs.
Please contact us if you need immediate assistance.
Please contact us (phone: 1-877-436-7274 or email: email@example.com),if you need immediate assistance.