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Western Blotting Kit, western blot

ONE-HOUR Western™ Advanced Kits

GenScript ONE-HOUR Western™ Advanced Kits are designed to detect proteins with very low concentration. The kits boost greater sensitivity with much less antibody. ONE-HOUR Western™ Advanced kits contain all the necessary reagents, buffers, nitrocellulose membranes, and HRP substrates for performing a Western blot.

Key Features

  • Superior sensitivity: The kits reveal the protein of very low amount.
  • Less Amount of Antibody Needed: As low as 0.5-2 μg of antibody is required to attain superior results

Examples

1. Comparison of the two ONE-HOUR Western™ Kits of different sensitivities using monoclonal antibodies:

Western Blotting Kit, western blot
Western blots of GST proteins using different ONE-HOUR Western™ Kits: Standard (L00205C) and Advanced (L00242). 33.0, 11.0, 3.60, 1.20, 0.41, 0.13 and 0.045 ng of GST proteins were loaded into Lanes 1, 2, 3, 4, 5, 6, and 7, respectively.

2. Comparison of the two ONE-HOUR Western™ Kits of different sensitivities using polyclonal antibodies:

Western Blotting Kit, western blot
Western blots of GST proteins using different ONE-HOUR Western™ Kits: Standard (L00204C) and Advanced (L00241). 25.0, 12.5, 6.25, 3.10, 1.50 and 0.78 ng of GST proteins were loaded into Lanes 1, 2, 3, 4, 5, and 6, respectively.

Ordering Information


Cat. No.
Name
Size
Price
Quantity
Technical Manual
L00241 ONE-HOUR Western™ Advanced Kit (Rabbit) 1 Kit (5 Assays) $125.00
ONE-HOUR Western Advanced Kit
L00242 ONE-HOUR Western™ Advanced Kit (Mouse) 1 Kit (5 Assays) $125.00

 
 
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