Bio-Layer Interferometry (BLI) is a label-free optical technique for measuring biomolecular interactions in real-time. It analyzes the interference pattern of white light reflected from an immobilized protein layer on the biosensor tip and an internal reference layer. Only molecules binding to or dissociating from the biosensor can shift the interference pattern, making BLI unique and ideal for protein binding, quantitation, affinity, and kinetics in crude samples.

Protein Insect


Outstanding Capability for Complex Protein
One Stop Solutions
Advanced Expression Optimization Technology
Large Capacity
Capacity for large inserts > 400kDa
Multiple Sample Types
Capacity for large inserts > 400kDa
Accept Crude Samples


Bio-Layer Interferometry (BLI) vs. Surface Plasmon Resonance (SPR)

BLI Specifications

  • Label-free detection, real-time data, high-throughput
  • Less reference channel required
  • Low sensitivity for samples with low Koff values
  • Crude sample compatibility
  • Sample can be recovered if needed
  • Fluidic-free system so less maintenance needed

SPR Specifications

  • Label-free detection, real-time data, high-throughput
  • Measures a wide range of on rates, off rates affinities
  • High cost
  • Ongoing fluidic maintenance

Service Details

For customers who wish to establish the best possible protocol for their project and then scale up, GenScript recommends using the sophisticated platform to explore different conditions for the expression of target proteins.

Services Sepecifications Amount (Pairs) Timeline (Wks) Deliverables
Antigen affinity measurement-kinetic (5 concentrations) 1-4 1 Analysis Report
5-24 1-2
25-96 2-3
>96 3
Antigen affinity measurement-affinity ranking (1 concentration) 1-4 1
5-24 1
25-96 1-2
>96 2-3

1. No additional capture sensor cost, if ProA, AHC2, AMQ are involved.
2. No additional method development cost, like optimization of ligand quantity, analyte concentration range, time of association and dissociation, etc.

Case Studies

Get in Touch
with GenScript Custom
Protein Services

Get in Touch with GenScript Custom Protein Services
Please enter a valid first name.
Please enter a valid last name.
Please enter a valid email name.
Please select a valid industry.
Please select a valid country.
Please enter a valid Zipcode.
Please enter a valid institution/company.
Please enter a message in the textarea.

Related Services

High Throughput

Designed for high-throughput screening, starting at 5 BDs

Learn More

Yield up to gram, starting at 10 BDs

Learn More
High Performance

Yield up to kg, from 6-7 weeks

Learn More
TurboCHOTM Stable Cell Pool & Cell Line

High expression level with ECACC-licensed CHO-K1 cell

Learn More

Quotation and Ordering

Our customer service representatives are available 24 hours Monday through Friday. You may contact us anytime for assistance. Orders can be placed online, over the phone, by email or fax. Please have your payment information (credit card, purchase order or bank information) ready.

Click the quote button, provide service information and submit
Our Technical Account Manager will contact you with 24 hours to finalize quote
Your review and approve final order details and price
You provide credit card/PO information and order is placed

Project is initiated inmediately
Our Project Manager will be in contact with you to provide updates


Do you like the current new website?