The sample demonstration with TScan utilized 6 high-copy plasmid samples grown in 200mL LB medium. After overnight growth, the bacterial pellet was centrifuged, and the wet pellet was weighed before loading onto the AmMag Quatro for purification. The final samples were eluted with water.
Sample ID | Wet pellet(gram) | Culture Volume(mL) | Concentration(ng/μL) | Ratio 260/280 | Ratio 260/230 | Elution Volume |
---|---|---|---|---|---|---|
1 | 1.33g | 200 | 583.00 | 1.91 | 2.09 | ~800μL |
2 | 1.27g | 200 | 588.10 | 1.92 | 2.07 | ~800μL |
3 | 1.28g | 200 | 545.75 | 1.91 | 2.07 | ~800μL |
4 | 1.2g | 200 | 525.95 | 1.93 | 2.00 | ~800μL |
5 | 1.05g | 200 | 429.60 | 1.91 | 2.00 | ~800μL |
6 | 1.29g | 200 | 567.70 | 1.91 | 2.06 | ~800μL |
Table 1: Purified Plasmid Sample Results from TScan Therapeutics Demonstration, including DNA Concentration and QC Metrics.