Anti-idiotype Antibody Development Services
Anti-idiotype antibody is an antibody against the antigen binding site (complementarity determining region, CDR region) of another antibody. Tracking antibody drug in biological fluid, such as serum from patients receiving antibody drug, requires the differentiation of the administered antibody drug from naturally-occurring endogenous antibodies. As anti-idiotype (anti-ID) antibody is specific to the unique variable region of the antibody drug, it has become a powerful tool for antibody drug pharmacokinetics (PK), pharmacodynamics (PD) and immunogenicity studies.
GenScript has extensive experience in developing highly sensitive and highly specific anti-idiotypic antibodies to support your antibody drug development. A major challenge resides in the anti-idiotype antibody development is the very low percentage of anti-ID antibody among other naturally-occurring endogenous antibodies in antibody drug immunized animals. Therefore, the appropriate immunization, purification and hybridoma screening strategies are critical for successful anti-ID polyclonal and monoclonal antibody development. At GenScript, our scientists utilize our proprietary technologies to ensure the high specificity and affinity of anti-idiotypic antibody, including special adjuvant and immune mediators, super-sensitive hybridoma screening, and optimal clone selection processes. We have accomplished 100% success rate of anti-ID antibody development for our clients.
Features of GenScript's Anti-ID Antibodies
- High specificity and affinity
- Fast turnaround time: 2-3 months
- Proven track record – anti-ID antibody development success rate of 100%
- Optimal clone selection: antigen ligand blocking, epitope binning and antibody pairing
- Availability of both polyclonal and monoclonal anti-idiotype antibody packages
- Readily integrated downstream antibody drug PK/PD and immunogenicity assay development
Anti-idiotype Antibody Packages
Services |
Anti-idiotype monoclonal antibody |
Anti-idiotype polyclonal antibody |
|---|---|---|
Starting material |
Target antibody drug 2-3mg |
Target antibody drug 20 mg or more |
Work flow |
 |
 |
QC |
Cross-reactivity with control IgG <10% |
Cross-reactivity with control IgG <10% |
Deliverables |
Hybridoma cell lines/Ab sequences, supernatants and purified anti-ID antibody (optional) |
0.5-3mg purified anti-ID antibody/rabbit |
See detailed anti-idiotype antibody development protocol here.
Of note, GenScript offers the development of both polyclonal and monoclonal anti-ID antibodies. Polyclonal anti-ID antibodies from goats or rabbits are suitable when a lower amount of antibodies are needed within a short time, e. g. for pre-studies. If the anti-idiotype antibodies will be used in long-term studies under validated conditions, then monoclonal anti-ID antibodies are recommended.
Quotation and Ordering
Our customer service representatives are available 24 hours a day, Monday through Friday to assist you.