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Anti-idiotype Antibody Services

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High Specificity and Sensitivity

Downstream Immunoassay Development Service

Upgraded! Anti-idiotype Antibody Services

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An anti-idiotype antibody (anti-ID Ab, see the schematic diagram) binds to the idiotype of another antibody, usually an antibody drug. An idiotype can be defined as the specific combination of idiotopes present within an antibodies complement determining regions. Since anti-ID Abs are capable of binding to antibody drugs within biological fluids, they are commonly used in preclinical setting for antibody drug pharmacokinetics (PK) and pharmacodynamics (PD). Due to the similarity between anti-ID Abs and anti-drug antibodies, anti-ID Abs are also commonly used as a reference standard for antibody drug immunogenicity(immune response, IR) studies.

GenScript has 14 years experience in developing of highly sensitive and specific polyclonal and monoclonal anti-ID Abs to support your antibody drug development. We try to work as closely as possible with our customers to understand their specific downstream application in order to generate their perfect anti-ID. See our case studies

Anti-Idiotype Antibodies

Type	1. Antigen-blocking	2. Non-blocking	3. Complex-specific
Schematic diagram
Feature	Paratope-specific	Not Paratope-specific	Partially paratope-specific
Antigen binding	Inhibitory	Not inhibitory	Not inhibitory
Application	Detect free drug only	Detect total drug (free and bound)	Detect bound drug typically
Selection Guideline	Type 1 or type 2 could be applied to detect antibody drug when having a cell-bound drug target e.g. PD1/PDL1 Type 1 & type 2 may both be needed when having a secret drug target e.g. TNF

Features of GenScript Anti-ID Antibodies Service

Highest Possible Affinity

Perfect for picomolar-level immunoassay sensitivity
Guaranteed Anti-ID Pair

Perfect for ELISA kit development
Stable & Easy Scale Up

Sequence delivery & gram level recombinant Ab supply

Anti-ID Package Details

Rodent mAb

Package	Starting Material	Type	Anti-ID Pair	Time (months)	Deliverable
Rodent anti-ID mAb development	≥ 2 mg target antibody drug (customer submit, purity > 85%) 0.5 mg Isotype control or human IgG (GenScript provide, purity > 85%)	1/2	N	3-4	1-2 mL supernatant/parental clone 5 mL final supernatant/subclone Hybridoma cell lines 2-5 mg purified Ab/clone(optional) Anti-ID pair and preliminary sandwich ELISA sensitivity report (optional) COA report
		1/2	Y	4-5
		1&2	Y*	4-5
QC standard: ELISA titer ≥ 1: 64,000; Cross rate with negative control ≤ 5%

* : At least one pairing anti-ID Ab (any kind) is guaranteed to delivery
Note: Complex-specific anti-ID Ab need to be further inquired

Rabbit mAb

Package	Starting Material	Type	Anti-ID Pair	Time (months)	Deliverable
Rabbit anti-ID mAb development	≥ 2 mg F(ab)2 , ≥ 3 mg Target antibody drug (customer submit, purity > 85%) 1 mg Isotype control or human IgG (GenScript provide, purity > 85%)	1/2	N	5-6	Small scale affinity purification after 3^rd immunization for in-house detection (optional) 2 mL supernatant/positive clone 5 mL final supernatant/positive clone 40 mL antiserum/rabbit or 2 mg purified pAb anti-ID (optional) Ab sequence report 1-10 mg recombinant Ab/clone (optional) Anti-ID pair and preliminary sandwich ELISA sensitivity report (optional) COA report
		1/2	Y	6-7
		1&2	Y*	6-7
QC standard: ELISA titer ≥ 1: 64,000; Cross rate with negative control ≤ 5%

* : At least one pairing anti-ID Ab (any kind) is guaranteed to delivery
Note: Complex-specific anti-ID Ab need to be further inquired

Rabbit pAb

Package	Starting Material	Time (months)	Deliverable
Rabbit anti-ID pAb development	≥ 45 mg Target antibody drug (customer submit, purity > 90%) ≥ 30 mg Isotype control-human IgG (GenScript provide, purity > 90%)	2	Small scale affinity purification after 3^rd immunization for in-house detection (optional) At least 4 mg affinity purified pAb COA report
QC standard: ELISA titer ≥1: 64,000; Cross rate with negative control ≤10%

PK/IR Kit

Package	Starting Material	Time (months)	Deliverable
PK/IG ELISA Kit Development	≥ 0.5 mg antibody drug, ≥ 1 mg detection antibody & ≥ 1mg capture antibody (customer submit, purity > 90%)	2	Feasibility report Kit development final report 0.5 mg Conjugated capture antibody and detection antibody 10 final kits in 96-tests format and kit specification
QC standards: Intra-assay precision ≤ 5%, Inter-assay precision≤ 10%, Recovery range between 100 ± 15%

PK/IR Kit Parameters

Case study: PK sandwich ELISA kit parameters

Features	Specification
Sensitivity	0.295 ng/ml
Detection Range	1.5625-100 ng/ml
Precision	Inter-assay： 6.51%; Intra-assay： 3.50%.
Recovery	Recovery range of this kit was between 85%-115%.
Test Samples	Human serum/plasma, mouse serum/plasma
Expiration date	Six months
Convenience	All reagents and buffers for the test are include in the kit The test could be completed within 2 hours

Case study: Immunogenicity bridging ELISA kit parameters

Features	Specification
Sensitivity	0.2056 ng/ml
Detection Range	1.5625-100 ng/ml
Precision	Inter-assay：3.74%; Intra-assay： 2.43%.
Recovery	Recovery range of this kit was between 85%-115%.
Test Samples	Human serum/plasma (EDTA), mouse serum, Rat serum/plasma (heparin), Rabbit serum/plasma (heparin)
Expiration date	Six months
Convenience	All reagents and buffers for the test are include in the kit The test could be completed within 2 hours

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