|Synonyms||Mouse Anti-GAPDH mAb;|
|Description||Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH) is an enzyme of ~37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. In addition to this long established metabolic function, GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation of apoptosis, and ER to Golgi vesicle shuttling.|
|Immunogen||Purified pig GAPDH|
|Subclass||Mouse Monoclonal (IgG1)|
|Concentration||1 mg/ml in PBS, pH 7.4 with 0.05% sodium azide, frozen liquid.|
|Specificity||Mouse Anti GAPDH is known to react with GAPDH form humans, cows, pigs, mice, rats and other mammals. It also recognizes avian GAPDH.|
|Species Reactivity||Human, cow, pig, rat and other mammals, avian|
|Storage||The product will remain stable for one year after the date of shipment if stored at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Aliquot will remain stable for three months if stored at 4°C.|
|Western blot at dilutions of 1:1,000 and immunofluorescence experiments at 1:100 dilutions respectively. It is very useful as a loading control for quantitative Western blotting.
|Technical Manual||ELISA Protocol(pdf)|
Western Blot Protocol(pdf)
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