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Catalog Products » Antibodies » Epitope Tag Antibodies » GST-tag Antibody, pAb, Rabbit

GST-tag Antibody, pAb, Rabbit

Synonyms Rabbit Anti-GST-tag pAb;
Description GenScript Rabbit Anti-GST-tag Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography. The immunogen used is a recombinant version of glutathione-S-transferase from Schistosoma japonicum. Glutathione-S-transferase is an epitope tag in generating fusion proteins. Antibodies specific to fusion proteins are useful in monitoring protein expression in Western Blots as well as immunoaffinity purification of proteins expressed in E. coli. The glutathione-S-transferases are a family of enzymes that catalyze the addition of the tripeptide glutathione to endogenous and xenobiotic substrates which have electrophilic functional groups. They play an important role in the detoxication and metabolism of many xenobiotic and endobiotic compounds. The glutathione adducts produced have increased solubility in water and are subsequently enzymatically degraded to mercapturates and excreted.
Host Species Rabbit
Antigen Species Schistosoma japonicum
Conjugation Unconjugated
Immunogen Recombinant Glutathione-S-Transferase from Schistosoma japonicum
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 0.5 mg/ml in PBS, pH 7.4 with 0.02% sodium azide
Reconstitution Reconstitute the lyophilized antibody with deionized water (or equivalent) to the final concentration of 0.5mg/ml.
Specificity This antibody can be used to detect GST-fusion proteins in almost expression systems including bacterial expression system, mammalian expression system and other systems.
Species Reactivity Schistosoma japonicum
Storage The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.5 µg/ml
Western blot: 0.1-1 µg/ml
Western Blot Using ONE-HOUR WesternTM Kit: For quick results, GenScript ONE-HOUR WesternTM Kit L00204C is recommended. 10 µg of this antibody is mixed with 10 ml of WB solution for 8 cm x 8 cm membrane.
Other applications: user-optimized

Cat. No. Product Name Quantity Price
Glutathione S-Transferase - 1 mg
GST tag ELISA Detection Kit - 1 kit (1x96 well)
GST Fusion Protein Purification Kit - 1 kit
Glutathione Resin - 10 ml
GST-tag Antibody [HRP], pAb, Goat - 40 ug
THETM GST Antibody [Biotin], mAb, Mouse - 200 ug
THETM GST Antibody [HRP], mAb, Mouse - 100 ug
THETM GST Antibody, mAb, Mouse - 100 ug
THETM GST Antibody, mAb, Mouse - 200 ug
THETM GST Antibody, mAb, Mouse - 500 ug
GST-tag Antibody [Biotin], pAb, Rabbit - 40 ug
GST-tag Antibody [HRP], pAb, Rabbit - 40 ug
Goat Anti-Rabbit IgG Antibody (H&L) [HRP], pAb - 1 mg

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