|Synonyms||Goat Anti Rabbit IgG (H&L) [HRP]; Anti Rabbit IgG (H&L) [HRP]|
|Description||GenScript Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody is highly purified from goat antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase. It reacts with rabbit IgG heavy and light chains.|
|Conjugation||Horseradish Peroxidase (HRP)|
|Immunogen||Full-length rabbit IgG (purified)|
|Concentration||1 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal|
|Reconstitution||Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 1.0 mg/ml.|
|Specificity||This antibody specifically recognizes rabbit IgG and other species are not tested.|
|Storage||The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.|
|GenScript Goat Anti-Rabbit IgG (H&L) [HRP] is suitable for immunoblotting (western and dot blots), ELISA, immunoperoxidase electron microscopy, immunohistochemistry, and other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
The investigator must determine the ideal working concentration for each specific application. The ideal working concentration must take into account such factors as secondary antibody affinity, antigen concentration, sensitivity of the detection method, temperature, and the length of the incubations. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 1:1,000-1:20,000
Other applications: user-optimized
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