Goat Anti-Rabbit IgG Antibody (H&L) [HRP], pAb Antibody

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Overview

Synonyms	Goat Anti Rabbit IgG (H&L) [HRP]; Anti Rabbit IgG (H&L) [HRP]
Description	GenScript Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody is highly purified from goat antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase. It reacts with rabbit IgG heavy and light chains.
Host Species	Goat
Antigen Species	Rabbit
Conjugation	Horseradish Peroxidase (HRP)
Immunogen	Full-length rabbit IgG (purified)
Purification	Immunoaffinity chromatography
cannot ship to canada	cannotShipToCanada

Properties

Subclass	Goat IgG
Concentration	1 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal
Reconstitution	Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 1.0 mg/ml.
Specificity	This antibody specifically recognizes rabbit IgG and other species are not tested.
Species Reactivity	Rabbit
Storage	The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

GenScript Goat Anti-Rabbit IgG (H&L) [HRP] is suitable for immunoblotting (western and dot blots), ELISA, immunoperoxidase electron microscopy, immunohistochemistry, and other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
The investigator must determine the ideal working concentration for each specific application. The ideal working concentration must take into account such factors as secondary antibody affinity, antigen concentration, sensitivity of the detection method, temperature, and the length of the incubations. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 1:10,000-1:50,000
Western blot: 1:1,000-1:20,000
Other applications: user-optimized

Technical Manual

ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

Related Products

Cat. No.	Product Name	Price
A00131	Goat Anti-Rabbit IgG Antibody (H&L), pAb - 1 mg	$50.00
A00160	Goat Anti-Mouse IgG Antibody (H&L) [HRP], pAb - 1 mg	$79.00
A00166	Goat Anti-Human IgG Antibody (H&L) [HRP], pAb - 1 mg	$79.00
A00178	Donkey Anti-Goat IgG Antibody (H&L) [HRP], pAb - 1 mg	$79.00

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