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ABCA1 Antibody, mAb, Mouse

*This product has been discontinued! *
Synonyms Mouse Anti-ABCA1 mAb; Anti ABCA1
Description This membrane-associated protein is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extracellular and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. This protein functions as a cholesteral efflux pump in the cellular lipid removal pathway. Mutations in this gene have been associated with Tangiers disease and familial high-density lipoprotein deficiency.
Host Species Mouse
Antigen Species Human
Conjugation Unconjugated
Immunogen Recombinant fragment, corresponding to amino acids 1800-2260 of Human ABCA1
Purification Immunoaffinity purification

Subclass Mouse monoclonal IgG1
Clone ID A00121.01
Concentration 1 mg/ml in PBS, pH 7.4 with 0.05% sodium azide, frozen liquid
Specificity Recognize specifically ABCA1 from human, mouse, and chicken
Species Reactivity Human, mouse, and chicken
Storage The antibody will remain stable for 1 year from the date of shipment if stored at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Aliquots will remain for three months if stored at 4°C.

Starting concentration for WB and ELISA are 1:1000, for IP and IHC are 1:100. This antibody has been tested with direct ELISA, where a 96-well plate was coated with recombinant ABCA1 C-terminal protein. The antibody was then added to the wells and a goat anti-mouse IgG HRP conjugate was used for colour development. For ABCA1 quantification, a pair of anti-ABCA1 antibodies should be used to formulate a Sandwich ELISA and with a presence of an ABCA1 protein Standard. Optimal dilution to be determined by the end user.
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

Frank PG, et al. Caveolin-1 and regulation of cellular cholesterol homeostasis. Am. J. Physiol. Heart Circ. Physiol. Aug 2006; 291(2): H677-686.

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