|Description||Thioredoxin (Trx) is small, multi-functional protein with oxidoreductase activity. It is a 12 kD redox protein with disulphide oxidoreductase activity. It has been used as an epitope tag in expression vectors.
GenScript Trx-tag Antibody, pAb, Goat is developed in goat using purified recombinant Trx-tag fusion protein. It is suitable for detecting fusion proteins that contain a Trx tag.
|Immunogen||Purified recombinant Trx-tag fusion protein|
|Concentration||0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.|
|Reconstitution||Reconstitute the lyophilized powder with deionized water (or equivalent) to antibody concentration of 0.5 mg/ml.|
|Specificity||GenScript Trx-tag Antibody, pAb, Goat specifically reacts with fusion proteins containing Trx epitope tags.|
|Species Reactivity||E. coli, human|
|Storage||The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.|
|Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1 µg/ml
Other applications: user-optimized
|Technical Manual||ELISA Protocol(pdf)|
Western Blot Protocol(pdf)
Western blot analysis of Trx-tagged fusion protein using Trx-tag Antibody, pAb, Goat (GenScript, A00129, 0.1 µg/ml)
The signal was developed with Donkey Anti-Goat IgG Antibody (H&L) [HRP], pAb (GenScript, A00178, 1:15,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221)
Predicted Size: 35 KD
Observed Size: 35 KD
|A00178||Donkey Anti-Goat IgG Antibody (H&L) [HRP], pAb||