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MARCKS Antibody, pAb, Rabbit

*This product has been discontinued! *
Synonyms Rabbit Anti-MARCKS pAb; Anti MARCKS
Description Myristoylated alanine-rich C kinase substrate (MARCKS) translocates from the cytosol to the plasma membrane while mononucleated myoblasts fuse to form multinucleated myotubes. Rabbit anti MARCKS is an affinity purified rabbit polyclonal and used for neuroscience and cancer research. MARCKS is a member of the calmodulin binding protein family, is localised to the plasma membrane, forming cross-links with actin filaments. Phosphorylation by protein kinase C at key serine residues results in inhibition of binding to actin, phosphatidyl serine and calmodulin. MARCKS is thought to be involved in mitogenesis, phagocytosis and membrane trafficking.
Host Species Rabbit
Antigen Species Rat
Conjugation Unconjugated
Immunogen Full length recombinant MARCKS (Myristoylated alanine rich C Kinase substrate) expressed in and purified from E. coli.
Purification Immunoaffinity purified

Subclass Rabbit IgG
Concentration 1 mg/ml in PBS, pH 7.4 with 0.05% sodium azide, frozen liquid
Specificity Myristoylated alanine rich C kinase substrate
Species Reactivity Rat
Storage Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Aliquot will be stable at 4°C for 3 months.

Immunofluorescence: 1:250-1:500
ABC or other enzyme linked immunocytochemical procedures: 1:500
Western blot: 1:1,000
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

Kim SS, et al. Involvement of protein phosphatase-1-mediated MARCKS translocation in myogenic differentiation of embryonic muscle cells. J. Cell. Sci. Jun 2002; 115(Pt 12): 2465-2473.

Gallant C, et al. MARCKS is a major PKC-dependent regulator of calmodulin targeting in smooth muscle. J. Cell. Sci. Aug 2005; 118(Pt 16): 3595-3605.

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