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MAP2 Antibody, pAb, Chicken

*This product has been discontinued! *
Synonyms Chicken Anti MAP2; Anti MAP2
Description Microtubules are 25 nm diameter protein rods found in most kinds of eukaryotic cells. They are polymerized from a dimeric subunit made of one a subunit and one b tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein t (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5. MAP2 is made up of two ≈280,000 Da apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ≈70,000 Da on SDS-PAGE gels. Antibodies to MAP2 are excellent markers on neuronal cells, their perikarya and neuronal dendrites. In contrast t (tau) is found predominantly in neuronal axons. Dendrite specific MAP found specifically in dendritic branching of neuron. This antibody does not bind to protein A or protein G.
Host Species Chicken
Antigen Species Human
Conjugation Unconjugated
Immunogen Recombinant MAP2
Purification Immunoaffinity purification

Subclass Chicken IgY
Concentration 1 mg/ml in PBS, pH 7.4 with 0.05% sodium azide, frozen liquid
Specificity MAP2 (Microtubule Associated Protein 2) from human, rat, mouse and bovine
Species Reactivity Human, rat, mouse and bovine
Storage Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Aliquot will be stable at 4°C for 3 months.

Immunofluorescence: 1:5,000
Western blot using chemiluminescence: 1:50,000 or lower
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

Hirokawa N. 270K microtubule-associated protein cross-reacting with anti-MAP2 IgG in the crayfish peripheral nerve axon. J. Cell Biol. Jul 1986; 103(1): 33-39.

Izant JG, McIntosh JR. Microtubule-associated proteins: a monoclonal antibody to MAP2 binds to differentiated neurons. Proc. Natl. Acad. Sci. USA. Aug 1980; 77(8): 4741-4745.

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